色谱2025，Vol.43Issue(2)：139-147,9.DOI:10.3724/SP.J.1123.2024.02015
基于非靶向代谢组学的缺血性脑卒中生物标志物的分析
Analysis of ischemic stroke biomarkers based on non-targeted metabolomics
摘要
Abstract
Biomarkers for ischemic stroke(IS)are yet to fulfill clinical requirements.This study used non-targeted metabolomics to investigate differential metabolites and metabolic pathways in plasma and brain tissue following IS,with the aim of identifying new potential bio-markers and therapeutic targets.Twelve Tibetan miniature pigs were randomly assigned to a model-or sham-operation group.An electrocoagulation-based anterior temporal approach was employed to occlude the middle cerebral artery,thereby creating a model for IS.Plasma and brain tissue samples were collected 36 h post-surgery and analyzed using liquid chromatogra-phy-mass spectrometry.Principal component and partial least squares discriminant analyses were used to screen for differential metabolites and exclude exogenous metabolites at p＜0.05.Compounds were classified according to the HMDB(Human Metabolome Database),and sub-jected to KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway and VIP(variable im-portance in projection)analyses.Plasma metabolomics revealed that 86 metabolites were up-regulated while 149 were downregulated,with(Z)-3-oxo-2-(2-pentenyl)-1-cyclopentylacetic acid,transs-cinnamic acid and cinnamoylglycine determined to be significant metabolites.Fifty-eight differential metabolites were upregulated in brain tissue and 53 were downregulated,with 2,3-dihydroflavon-3-ol,guanidinoacetic acid(GAA),N-acetyl-D-tryptophan,oxidized glutathi-one,2-hydroxyquinoline,and N-acetyl-L-aspartate(NAA)identified as significant metabolites.Organic acids and derivatives,lipids and lipid-like molecules,organoheterocyclic compounds,and organic oxygen compounds were found to be common compound categories among the top five types of compound in both plasma and brain tissue.Common metabolic pathways in plasma and brain tissue include amino acid metabolism,digestive system,cancer overview,and lipid metabolism pathways,with the(Z)-3-oxo-2-(2-pentenyl)-1-cyclopentylacetic acid,GAA,oxi-dized glutathione,and NAA metabolites serving as potential biomarkers.This study provides a theoretical foundation for the early screening and development of clinical treatment strategies for IS.关键词
缺血性脑卒中/非靶向代谢组学/生物标志物/血浆/脑组织Key words
ischemic stroke(IS)/non-target metabolomics/biomarkers/plasma/brain tissue分类
化学化工引用本文复制引用
徐菲,刘天平,关雅今,郝伟超,温鼎声,谢水林,别亚男..基于非靶向代谢组学的缺血性脑卒中生物标志物的分析[J].色谱,2025,43(2):139-147,9.基金项目
广东省基础与应用基础研究基金项目(2022A1515110078) （2022A1515110078）
广州市基础与应用基础研究项目(202201010358,202201010139) （202201010358,202201010139）
佛山市高新区高技术产业化与创业团队项目(2120197000014) （2120197000014）
国家自然科学基金项目(82203847,82301476).Guangdong Basic and Applied Basic Research Fund Project(No.2022A1515110078) （82203847,82301476）
Basic and Applied Basic Research Project of Guangzhou(Nos.202201010358,202201010139) （Nos.202201010358,202201010139）
High Tech Industrialization and Entrepreneurship Team Project Funding of Gaoxin District,Foshan City(No.2120197000014) （No.2120197000014）
National Natural Science Foundation of China(Nos.82203847,82301476). （Nos.82203847,82301476）
