色谱2025，Vol.43Issue(2)：177-184,8.DOI:10.3724/SP.J.1123.2023.11021
自适应精准控温型液相色谱柱温箱的设计与评价
Design and evaluation of a liquid chromatographic column oven with adaptive and precise temperature control
摘要
Abstract
High performance liquid chromatography(HPLC)is a key analytical technique that is used in a number of fields.Improving the separation efficiency,stability,and universality of HPLC has been a continuing analytical-chemistry focus.In chromatographic separation,factors such as the composition and ratio of the mobile phase,the type of stationary phase,and the di-mensions of the chromatographic column significantly affect the separation efficiency.In addi-tion,the temperatures of the chromatographic column and mobile phase are also important for achieving separation.The column oven is usually used to stably control the column temperature in the HPLC separation system.Indeed,highly accurate temperature control ensures superior separation performance,short analysis times,and repeatability.In this study,we innovatively improved the traditional column oven by combining a variety of temperature-control algorithms to deliver continuous and highly accurate temperature control in the wide 4-90 ℃ range,and by exploring a new chromatographic-method development route.Instead of focusing on the complex hardware system,we optimized the software to achieve highly stable and accurate(±0.1 ℃)temperature control.Temperature-control performance was further improved by op-timizing the structure of the thermal insulation and employing reliable and environmentally friendly thermal-insulation materials.Additionally,the thermal conduction of the heat-source device is discussed based on the heat-transfer principle with the aim of improving the perform-ance of the column oven.The improved column oven delivered significantly enhanced chroma-tographic-separation repeatability and stability thereby reliably guaranteeing the development of highly efficient chromatographic analysis methods.关键词
精准控温/算法/传热原理/液相色谱/柱温箱Key words
precise temperature control/algorithm/heat transfer principle/liquid chromatog-raphy/column oven分类
化学化工引用本文复制引用
任兴发,邵锋伟,吴勇,廖信明,王卓,周林娟,何旭鸿,张维冰..自适应精准控温型液相色谱柱温箱的设计与评价[J].色谱,2025,43(2):177-184,8.基金项目
国家重点研发计划资助项目(2021YFF0701900) （2021YFF0701900）
上海市2020年度"科技创新行动计划"科学仪器领域项目(20142200900).National Key Research and Development Program(No.2021YFF0701900) （20142200900）
Shanghai 2020"Science and Technology Innovation Action Plan"Scientific Instrument Field Project(No.20142200900). （No.20142200900）
