生态学杂志2025，Vol.44Issue(2)：417-424,8.DOI:10.13292/j.1000-4890.202502.030
长白山阔叶红松林土壤真菌和细菌N2O排放对施氮和降水变化的响应
Responses of soil fungal and bacterial N2O emissions to nitrogen fertilization and precipitation changes in a broad-leaved Korean pine forest in Changbai Mountains,China
摘要
Abstract
Combined with the antibiotic inhibition technique,we examined the responses of soil fungal and bacteri-al N2O emissions in a laboratory culture experiment.Soil samples were collected from fixed plots with long-term(eightyears)nitrogen application and precipitation reduction in a broad-leaved Korean pine forest in Changbai Mountains.Results showed that soil N2O emissions were 0.79 nmol·g-1·h-1 in the control.Nitrogen application,precipitation reduction,and simultaneous precipitation reduction and nitrogen application significantly enhanced soil N2O emissions,which were 3.55,1.84 and 1.84 nmol·g-1·h-1,respectively.There was a significant interaction between precipitation reduction and nitrogen application.Bacterial N2O emissions were affected by nitrogen applica-tion and precipitation reduction.Nitrogen application promoted bacterial N2O emissions,thereby promoting total soil N2O emissions.Fungal N2O emissions were mainly affected by precipitation reduction.Simultaneous precipitation reduction and nitrogen application promoted fungal N2O emissions,thereby promoting total soil N2O emissions.The increase in N2O emissions caused by precipitation reduction treatment was attributed to the interaction of bacteria and fungi.In the control,precipitation reduction,and nitrogen application treatments,N2O emissions were mainly contributed by bacteria,with relative contribution rates of 90.3％,68.2％and 91.1％,respectively.In the nitrogen application and precipitation reduction treatment,N2O emissions were mainly contributed by fungi,with a contribu-tion rate of 68.5％.Therefore,long-term nitrogen application under drought scenario would alter the main microbial species involved in soil N2O emission,directly affect the amount of N2O emissions from soil fungi and bacteria,thereby affecting total soil N2O emissions,which influence nitrogen cycling in forest ecosystems.关键词
土壤N2O排放/真菌/细菌/氮沉降/减水Key words
soil N2O emission/fungi/bacteria/nitrogen deposition/precipitation reduction引用本文复制引用
张蕾,雷馥歌,宿振浩,杨佳诺,张悦,郭忠玲,韩旭..长白山阔叶红松林土壤真菌和细菌N2O排放对施氮和降水变化的响应[J].生态学杂志,2025,44(2):417-424,8.基金项目
国家重点研发计划项目(2022YFF1300500)和全国大学生创新创业项目(120722145)资助. （2022YFF1300500）
