生态学杂志2025，Vol.44Issue(2)：451-459,9.DOI:10.13292/j.1000-4890.202502.021
施氮和高温胁迫对杜鹃幼苗根系构型和生理指标的影响
Effects of nitrogen application and high-temperature stress on root configuration and physiological indices of rhododendron seedlings
摘要
Abstract
We investigated the alleviating effects of nitrogen(N)application(0,0.46 and 0.92 g per plant)on Rhododendron'Wucai'ng'under different high temperature stresses(26 ℃/22 ℃,34 ℃/30 ℃ and 38 ℃/34 ℃),aiming to provide a scientific basis for enhancing plant stress resistance and adaptation to climate change.The results showed that there was a trend of fork-shaped branch structure formed in the roots of seedlings under high temperature stress.At 26 ℃/22 ℃,there was a significant increase in root bifurcation in the N fertilizer treatment of 0.46 g per plant,but a significant decrease in the N fertilizer treatments of 0 and 0.92 g per plant.Under the same level of N fertilizer,there were significant decreases in leaf area,total root length,root surface area,specific root length,specific surface area,and root-shoot ratio,but significant increases in root topology coefficient,soluble protein content,and free amino acid content in plants at 34 ℃/30 ℃ and 38 ℃/34 ℃,compared to that at 26 ℃/22 ℃.There were significant increases in leaf area,specific root length,root-shoot ratio,soluble protein content and free amino acid content of plants under high temperature stress in low N fertilizer treatment(0.46 g·plant-1),while there were significant decrease in leaf area,specific root length,root-shoot ratio,except for a significant increase in soluble protein content and free amino acids,in the high N fertilizer treatment(0.92 g·plant-1).The results of principal component analysis and membership function analysis showed that the best performance of seed-lings was recorded in the treatments of T3(26 ℃/22℃and 0.92 g·plant-1),T2(26 ℃/22℃and 0.46 g·plant-1)and T5(34 ℃/30 ℃ and 0.46 g·plant-1),with a score of 0.853,0.599 and 0.550,respectively,exhibiting better overall performance than that in the T1 control treatment(26 ℃/22℃and 0 g·plant-1).In con-clusion,high temperature stress would restrict root growth of seedlings and reduced soluble protein content.Appro-priate application of nitrogen fertilization(0.46 g·plant-1)could alleviate the inhibition in root growth under high temperature and enhance the resistance and adaptation of plants to high temperature.These findings provide theoret-ical guidance and reference for adopting fertilization in advance in the cultivation of Rhododendron to avoid summer heat damage with high temperature and promoting the application of excellent varieties of Rhododendron in gardens.关键词
杜鹃/氮素施肥/夏季高温热害/根系生长/生理特性Key words
Rhododendron/nitrogen fertilization/summer heat damage/root growth/physiological characteristics引用本文复制引用
曾鸣谦,吴丽君,周围,赵二毛,曹受金..施氮和高温胁迫对杜鹃幼苗根系构型和生理指标的影响[J].生态学杂志,2025,44(2):451-459,9.基金项目
湖南省林业厅科技创新项目(XLK201830)和湖南省教育厅优秀青年项目(22B0253)资助. （XLK201830）
