实用医学杂志2025，Vol.41Issue(3)：339-346,8.DOI:10.3969/j.issn.1006-5725.2025.03.005
miR-483-3p对乏氧/复氧诱导的心肌细胞凋亡和焦亡的作用研究
Effects of miR-483-3p on hypoxia/reoxygenation-induced apoptosis and pyroptosis in cardiomyocytes
摘要
Abstract
Objective To investigate the effects of miR-483-3p on hypoxia/reoxygenation(H/R)-induced apoptosis and pyroptosis of H9c2 cardiomyocytes and its possible mechanism.Methods Rat H9c2 cardiomyocytes were cultured in vitro,adeno-associated virus-infected H9c2 and the H/R model were constructed by triple-air incuba-tor,and the cells were randomly divided into blank control(Sham)group,model(H/R)group,AAV-miR-483-3p mimic+H/R(AAV-miR-483-3p)group,AAV-miR-483-3p negative control+H/R(AAV-NC)group.The growth status of cells in each group was observed using an inverted microscope;cell proliferation activity was detected by cell counting kit-8(CCK-8);LDH release by lactate dehydrogenase(LDH)kit;apoptosis rate by flow cytometry;apoptosis by notched end labeling(TUNEL).Western blot(WB)was used to detect the expression levels of IL-1β and GSDMD proteins in each group.Results Compared with the Sham group,the H/R group showed abnormal cell status and increased cell death,decreased cell activity,increased LDH release,increased apoptosis rate and apopto-sis level,and increased expression levels of IL-1β and GSDMD proteins(P<0.05);compared with the H/R group,the AAV-miR-483-3p group showed improved cell status and less cell death,increased cell proliferation activity,increased LDH release,and increased IL-1β and GSDMD protein expression levels(P<0.05).Compared with the H/R group,the AAV-miR-483-3p group showed improved cell status and less cell death,increased cell proliferation activity,decreased LDH release,decreased apoptosis rate and apoptosis level,and decreased expression of IL-1β and GSDMD proteins(P<0.05).Conclusion Over-expression of miR-483-3p can improve H/R-inducedH9c2 cardiomyocyte injury by enhancing cell activity and cell metabolism,and inhibiting apoptosis and cell charring.关键词
miR-483-3p/心肌缺血再灌注损伤/细胞凋亡/细胞焦亡Key words
miR-483-3p/myocardial ischemia-reperfusion injury/apoptosis/cellular pyroptosis分类
医药卫生引用本文复制引用
卢钰芬,郑孝明,韦少娟,陈礼琴,徐彤彤,吕祥威..miR-483-3p对乏氧/复氧诱导的心肌细胞凋亡和焦亡的作用研究[J].实用医学杂志,2025,41(3):339-346,8.基金项目
国家自然科学基金项目(编号:82160068,82260864) （编号:82160068,82260864）
