生态环境学报2025，Vol.34Issue(2)：233-246,14.DOI:10.16258/j.cnki.1674-5906.2025.02.006
纳帕海高寒退化草甸土壤细菌群落结构对氮添加的响应
Response of Soil Bacterial Community Structure to Nitrogen Addition in Degraded Napahai Alpine Meadow
摘要
Abstract
In this study,we aimed to explore the response mechanism of soil bacterial community composition and diversity under the background of increasing nitrogen deposition and conducted a short-term field simulation experiment with four nitrogen-level treatments:control(0 g·m-2·a-1),low(5 g·m-2·a-1),medium(10 g·m-2·a-1),and high nitrogen(15 g·m-2·a-1),in the Carex nubigena community in an alpine degraded Napahi meadow.High-throughput sequencing was used to systematically analyze the effects of plant and soil physicochemical properties on bacteria under nitrogen addition.The results showed the following:1)Nitrogen addition significantly affected the soil bacterial community composition at the phylum level.With an increase in nitrogen concentration,the relative abundances of Acidobacteriota,Actinomycetota,Methylomonas,Gemmatimonadota,and Nitrospira first increased and then decreased,with an increase of 1.05%to 38.24%compared with the control.The relative abundance of Proteobacteria and Firmicutes increased by 6.02%-90.24%compared to the control,while those of Chloromycota,Myxococcus,Bacteroidetes,and Verrucomicrobiota showed a decreasing trend of 14.25%-36.18%.At the genus level,Acidobacterium,Gemmatimonas,Vicinamibacter,and RB41 showed a trend of first increasing and then decreasing,Micrococcus and Xanthobacter showed a trend of first decreasing and then increasing,and Candidatus Solibacter showed an increase.2)Nitrogen addition significantly increased the aboveground plant biomass and decreased the plant diversity.The aboveground plant biomass increased by 65.4%,whereas the Shannon,Simpson,Margalef,and Pielou indices decreased by 13.9%-24.6%.Nitrogen addition significantly affected soil physicochemical properties.With the increase in nitrogen concentration,in contrast with the control,available potassium,organic matter,and available nitrogen increased by 7.04%-59.48%;total potassium and pH value decreased by 4.7%-18.18%;total and available phosphorus decreased by 1.67%-34.52%under the low nitrogen treatment and then increased.3)Changes in plant and soil environments significantly affected the bacterial community under nitrogen deposition.Structural equation analysis showed that changes in plant diversity indirectly regulated the composition and diversity of the bacterial community by affecting soil nutrients,whereas changes in the soil environment directly affected the bacterial community.Total soil nutrients,available nutrients,and pH were the main factors controlling bacterial community changes.Short-term nitrogen addition directly or indirectly regulates soil bacterial diversity and phylum composition by mediating changes in plant diversity and pH and affecting soil nutrient content.关键词
氮添加/高寒退化草甸/土壤细菌/多样性/群落结构/纳帕海Key words
nitrogen addition/alpine meadow/soil bacteria/diversity/community structure/Napahai分类
农业科技引用本文复制引用
孙煜佳,陆梅,赵旭燕,冯峻,刘国庆,郭础鸟,王明柳,黄敏超,陈志明..纳帕海高寒退化草甸土壤细菌群落结构对氮添加的响应[J].生态环境学报,2025,34(2):233-246,14.基金项目
国家自然科学基金地区科学基金项目(42067011) （42067011）
云南省中青年学术技术带头人后备人才项目(202205AC160047) （202205AC160047）
云南省应用基础研究面上项目(202001AT070113) （202001AT070113）
