新医学2025，Vol.56Issue(1)：20-26,7.DOI:10.12464/j.issn.0253-9802.2024-0306
Rictor/mTORC2信号促进Kras突变癌细胞的增殖和致瘤性
Rictor/mTORC2 signaling promotes proliferation and tumorigenicity of Kras mutant cancer cells
摘要
Abstract
Objective To explore the expression profile of Rictor/mTORC2 in Kras mutant tumor cells and its effect on cell proliferation and tumorigenicity.Methods The frequency of Kras and Rictor gene variations was investigated by using pan-cancer data from public databases.The co-expression relationship between Kras and Rictor gene variations and their respective gene expressions was analyzed using Pearson correlation analysis.Multiple cancer cell models with Kras mutation were used to down-regulate the expression of Rictor gene through antisense RNA sequence,and the effects of Rictor/mTORC2 signaling pathway on the proliferation and tumorigenicity of Kras mutant tumor cells were investigated through CCK8 and clonal formation assays,respectively.Results The variation frequencies of Kras and Rictor genes exceeded 90%and 30%,respectively,most commonly occurring in pancreatic cancer,lung cancer,colon cancer and breast cancer.Rictor gene detection results in a series of cell lines carrying Kras gene mutation and wild-type Kras gene were obtained.Pearson correlation coefficient showed that Kras and Rictor gene variations were positively correlated(both P＜0.05),and mRNA expression levels showed a common trend.Down-regulating Rictor gene expression could significantly inhibit the proliferation and tumorigenic ability of Kras mutant cancer cells(both P＜0.05).Conclusion The Rictor/mTORC2 signaling pathway is obviously activated in Kras mutant cancer cells,and can promote the proliferation and tumorigenicity of Kras mutant cancer cells.关键词
Kras/Rictor/Kras突变的癌细胞/增殖/致瘤性Key words
Kras/Rictor/Kras mutant cancer cell/Proliferation/Tumorigenicity引用本文复制引用
赖慧玲,陈淑琴..Rictor/mTORC2信号促进Kras突变癌细胞的增殖和致瘤性[J].新医学,2025,56(1):20-26,7.基金项目
国家自然科学基金(82303279) （82303279）
广东省基础与应用基础研究基金项目(2022A1515012401) （2022A1515012401）
