新医学2025，Vol.56Issue(1)：44-52,9.DOI:10.12464/j.issn.0253-9802.2024-0018
miR-205-5p启动子甲基化对子宫内膜异位症患者子宫内膜细胞功能调控的研究
Regulatory effect of miR-205-5p promoter methylation on endometrial stromal cell function in patients with endometriosis
摘要
Abstract
Objective To investigate the regulatory effect of miR-205-5p promoter methylation on endometrial stromal cell(ESC)function in patients with endometriosis(EMS).Methods Endometrial tissue samples were collected from patients pathologically diagnosed with EMS undergoing surgery in Zhujiang Hospital of Southern Medical University from October 2022 to December 2023.According to intraoperative and pathological results,all samples were divided into the control group(n=9,without EMS lesions)and EMS group(n=9,with EMS lesions).Primary ESCs were extracted and isolated.Methylation-specific polymerase chain reaction(MSP)was used to detect the methylation level of miR-205-5p promoter.RTPCR was used to evaluate the effect of demethylation on the expression level of miR-205-5p.The regulatory effect of demethylation on ESC function was detected by scratch wound healing,cell proliferation,cell apoptosis and Transwell assays,respectively.EMS mouse models were established by subcutaneous seeding of human endometrial debris.All mouse models were divided into the demethylation and control groups.Demethylating agents and normal saline were injected into abdominal cavity in these two groups,respectively.The lesion size and the expression level of miR-205-5p were observed in two groups.Results Normal ESC prompter was unmethylated.In the EMS samples,the miR-205-5p promoter was methylated or hypomethylated.After demethylation,the expression level of miR-205-5p in the ESC samples was significantly upregulated,the capability of cell migration,proliferation and invasion was significantly weakened,while the ability of cell apoptosis was significantly enhanced(all P＜0.05).Compared with the control group,the lesion size was decreased and the positive rate of miR-205-5p expression was increased in the demethylation group(both P＜0.05).Conclusion MiR-205-5p promoter methylation is related to the regulation of ESC function in patients with EMS,which is probably involved with the incidence and progression of EMS.关键词
子宫内膜异位症/miR-205-5p/甲基化/子宫内膜细胞Key words
Endometriosis/miR-205-5p/Methylation/Endometrial stromal cell引用本文复制引用
马菘,蔡朝霞,马颖..miR-205-5p启动子甲基化对子宫内膜异位症患者子宫内膜细胞功能调控的研究[J].新医学,2025,56(1):44-52,9.基金项目
广东省自然科学基金(2023A1515011688,2022A1515011880) （2023A1515011688,2022A1515011880）
南方医科大学珠江医院院长基金(yzjj2022ms18) （yzjj2022ms18）
