中国农学通报2025，Vol.41Issue(2)：43-48,6.
短期施肥对麦田土壤细菌群落组成和多样性的影响
Effects of Short-term Fertilization on Soil Bacterial Community Composition and Diversity in Wheat Fields
摘要
Abstract
To clarify the mechanism of the effect of short-term fertilization on the composition and diversity of soil bacterial communities in wheat fields,a field experiment was conducted with four treatments:no fertilization(T1),conventional fertilization(T2),recommended fertilization(T3)and organic substitution of 50％(T4),by using high-throughput sequencing to study the effects on soil bacterial community composition and diversity.The results showed that compared with T1 treatment,fertilization treatments decreased soil pH value,but T4 treatment delayed the downward trend of pH;T2,T3 and T4 increased the yield by 147.45％,144.68％and 98.41％,respectively.The T4 treatment increased the Shannon index and decreased the soil bacterial Chao index,but the differences were not significant.Fertilization increased the relative abundance of soil Proteobacteria,among which T2 was the highest,while fertilization decreased the relative abundance of Cyanobacteria,among which T4 was the highest.T2 enriched g_Roseateles,g_Rhodanobacter and g_Singulisphaera,T4 enriched g_Pseudoxanthomonas and o_Streptosporangiales.Cluster analyses showed that alkali-hydrolyzable nitrogen,available phosphorus,available potassium and organic carbon had similar effects on bacterial flora,but pH was different.Compared with T2,T4 slowed down the evolution of photoautotrophic flora to chemo-energy heterotrophic flora and decreased the abundance of nitrate respiration-related flora.Therefore,short-term organic substitution(50％)has the risk of reducing yield,but it was beneficial to maintain the stability of bacterial community in wheat field.N 150 kg/hm2 could be used as the recommended amount of fertilization.关键词
施肥/短期施肥/麦田/土壤/细菌多样性/细菌群落/功能预测/高通量测序Key words
fertilization/short-term fertilization/wheat fields/soil/bacterial diversity/bacterial community/functional prediction/high-throughput sequencing分类
农业科技引用本文复制引用
刘东海,戴志刚,梅亮贤,乔艳,张智,肖卓熙,李菲,胡诚..短期施肥对麦田土壤细菌群落组成和多样性的影响[J].中国农学通报,2025,41(2):43-48,6.基金项目
国家农业科技项目"农田智慧施肥项目"(20221805). （20221805）
