中国兽医科学2025，Vol.55Issue(2)：151-162,12.DOI:10.16656/j.issn.1673-4696.2024.0209
GP35蛋白在牛支原体中互作分子的挖掘
Exploration of interacting molecules with GP35 protein in Mycoplasma bovis
摘要
Abstract
GP35 protein was expressed in Mycoplasma bovis and the interacting molecules within the organism were screened by IP-MS.Following the expression of the interacting molecules and GP35 protein in a prokaryotic system,interaction validation was carried out.The results showed that IP-MS in M.bovis identified the SSB protein and the RmuC protein,the former being crucial in the DNA replication process and the latter related to DNA recombination.After the expression of GP35,SSB,and RmuC proteins in prokaryotes,pull-down assays confirmed the interactions between GP35 protein and SSB and RmuC proteins.Further analysis of the SSB protein may help elucidate the recombination mechanism mediated by GP35 protein in M.bovis.关键词
牛支原体/GP35重组酶/互作分子/同源重组Key words
Mycoplasma bovis/GP35 recombinase/interacting molecules/homologous recombination分类
农业科技引用本文复制引用
尹号,尤留超,黄蓉,韩瑞,高鹏程,支飞杰,马轲,付磊,储岳峰..GP35蛋白在牛支原体中互作分子的挖掘[J].中国兽医科学,2025,55(2):151-162,12.基金项目
国家自然科学基金面上项目(32373019) （32373019）
中国农业科学院科技创新工程项目(CAAS-ASTIP-JBGS-20210701) （CAAS-ASTIP-JBGS-20210701）
甘肃省重点人才项目(2021RCXM047) （2021RCXM047）
中国农业科学院兰州兽医研究所基本业务费-科研业务费项目(1102-31160042012) （1102-31160042012）
甘肃省陇原青年创新创业人才个人项目(2023LQGR55) （2023LQGR55）
