中国兽医科学2025，Vol.55Issue(2)：163-170,8.DOI:10.16656/j.issn.1673-4696.2025.0010
天冬氨酸蛋白酶在卵形巴贝斯虫侵染长角血蜱过程中的作用研究
Role of aspartic protease in the process of Babesia ovata infection of Haemaphysalis longicornis
摘要
Abstract
In this study,aspartic protease(AP)was used as a target gene to investigate its ability to inhibit the infection of Babesia ovata with Haemaphysalis longicornis,and the full length and coding sequence(CDS)are amplified by 5'/3'-RACE and PCR,and then designed siRNA interference primers based on the obtained sequences.An animal infection model was established by inoculating with Babesia ovata via the jugular vein,and after RNA interference(RNAi)with AP gene expression,the Haemaphysalis longicornis were placed on the surface of infected cattle.The infection rate of Babesia ovata in the ticks and tick eggs was assessed after feeding.The results indicated that the full length of the AP gene in Haemaphysalis longicornis is 1 497 base pairs,with a coding sequence of 1 176 base pairs.Notably,RNAi of this gene could reduce the expression of the AP by 81.91％(P=O.003＜0.01),which is statistically significant.In the process of infecting animals,the results of qRT-PCR showed that the Babesia ovata infection rate decreased in the group compared with blank control group and negative control group.This indicates that interference with Haemaphysalis longicornis AP can effectively inhibit the vertical transmission of Babesia ovata.Although this gene is highly conserved among tick species,there are still discernible differences in its nucleic acid sequences.Potential vaccine design targets can be identified within specific nucleotide positions,namely positions 626-644 or 257-275,which could serve as sites for blocking the transmission of Babesia ovata.Such targeted interventions may play a crucial role in suppressing the expression of the aspartic protease,thereby significantly hindering the vertical propagation of the pathogen and leading to a substantial reduction in infection levels.In conclusion,this research not only contributes to the understanding of the biological characteristics of Babesia ovata but also offers potential new strategies for disease control,ultimately providing more effective interventions for public health.关键词
长角血蜱/卵形巴贝斯虫/天冬氨酸蛋白酶/RNA干扰/RACEKey words
Haemaphysalis longicornis/Babesia ovata/aspartate protease/RNA interference(RNAi)/RACE分类
农业科技引用本文复制引用
罗金,殷宏,罗建勋,刘光远,张高峰,崔晶晶,柳志成,赵帅阳,任巧云,胡东生,曹立华,常国勤..天冬氨酸蛋白酶在卵形巴贝斯虫侵染长角血蜱过程中的作用研究[J].中国兽医科学,2025,55(2):163-170,8.基金项目
中国农业科学院科技创新工程项目(CAAS-ASTIP-2021-LVRI) （CAAS-ASTIP-2021-LVRI）
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
国家寄生虫资源库项目(NPRC-2019-194-30) （NPRC-2019-194-30）
