中国兽医科学2025，Vol.55Issue(2)：182-187,6.DOI:10.16656/j.issn.1673-4696.2025.0016
猪链球菌7型的分离鉴定及致病性研究
Isolation,identification and pathogenicity of Streptococcus suis type 7
摘要
Abstract
In order to investigate the pathogenicity of Streptococcus suis type 7,the bacteria were isolated and purified from diseased pigs in the Shandong peninsula.Four strains of type 7 were identi-fied by Gram staining,biochemical identification and PCR.The pathogenicity of S.suis type 7 was veri-fied by mouse infection test,the median lethal dose was determined,and clinical symptoms and histopathological sections were observed.The results showed that the bacteria appeared as round or oval pale translucent colonies on the blood plate,with β-hemolytic rings around the colonies.Gram staining was positive,and the colonies were arranged in single,multiple,or chain patterns.API20 strep biochemical identification results were Streptococcus genus,and PCR amplification of cps7 results were positive.Animal experiments showed the SS7-4 strain had the strongest pathogenicity to BALB/c mice,with a LD50 of 2.35 × 104 CFU/mL.The tissues of mice in the challenge group were severely swollen and bleeding,the edges of the organs were blunt and the color became dark red or black red.Histological ob-servations showed myocardial fiber rupture,abnormal increase in spleen trabeculae,obvious swelling of renal tubules,and severe congestion of lungs.The results of this experiment lay the foundation for further study of the pathogenic mechanism of S.suis type 7.关键词
猪链球菌7型/分离鉴定/动物试验/BALB/c小鼠/致病性Key words
Streptococcus suis type 7/isolation and identification/animal experiments/BALB/c/pathogenicity分类
农业科技引用本文复制引用
姜志康,韩先杰,郭皖露,陈超,柳宇,刘康,王贤文,郝炬源,刘刚..猪链球菌7型的分离鉴定及致病性研究[J].中国兽医科学,2025,55(2):182-187,6.基金项目
国家自然科学基金青年基金项目(32200151) （32200151）
山东省自然科学基金青年基金项目(ZR2022QC043) （ZR2022QC043）
山东省重大科技创新工程项目(2019JZZY010720) （2019JZZY010720）
山东省现代农业产业技术体系生猪创新团队资助项目(SDAIT-08-10) （SDAIT-08-10）
