中国兽医杂志2025，Vol.61Issue(2)：1-9,9.DOI:10.20157/j.cnki.zgsyzz.2025.02.001
江苏省、山东省和安徽省禽致病性大肠杆菌的分子流行病学调查
Molecular Epidemiological Investigation of Avian Pathogenic Escherichia coli in Jiangsu,Shandong,and Anhui Province
摘要
Abstract
To elucidate the molecular epidemiological characteristics of avian pathogenic Escherichia coli(APEC)in Jiangsu,Shandong,and Anhui provinces,this study collected 918 samples in 2023 from large-scale poultry farms.Samples included chicken feces,feather-deposited eggs,yolks,and thighs,bone marrow,joints,hearts,spleens,lungs,and intestinal contents from diseased or deceased chickens.APEC strains were isolated using selective media,and antimicrobial susceptibility was assessed via broth microdilution.Whole-genome sequencing was employed to analyze resistance and virulence genes,and multilocus sequence typing(MLST),O-antigen serotyping,and phylogenetic tree construction were performed to determine the epidemiological characteristics of the isolates.A total of 390 APEC strains were isolated,with an isolation rate of 42.48%.The isolates exhibited high resistance rates to ampicillin(91.03%,355/390),ceftiofur(82.82%,323/390),gentamicin(81.03%,316/390),and tetracycline(77.95%,304/390),and were sensitive to meropenem(0.51%,2/390)and colistin(3.08%,12/390).Predominant resistance genes included blaEC(98.97%,386/390),blaTEM(80.00%,312/390),aph(6)-Id(54.10%,211/390),erm(D)(98.46%,384/390),tet(A)(81.79%,319/390),and floR(63.33%,247/390).Virulence gene analysis revealed that all isolates carried at least four virulence genes.A total of 68 serotypes were identified,with the predominant serotypes being O78(11.03%,43/390),O180(7.95%,31/390),and O15(7.18%,28/390).A total of 71 sequence types(STs)were identified through MLST,with the main ST types being ST117(15.9%,62/390),ST155(8.46%,33/390),and ST162(8.2%,32/390).Phylogenetic analysis showed that isolates formed four evolutionary clades,with substantial genetic divergence among isolates from different provinces.These findings indicate that the predominant APEC serotypes in Jiangsu,Shandong,and Anhui provinces are O78,O180,and O15,with ST117 being the primary sequence type.The isolates exhibited high prevalence of resistance and virulence genes.Continued epidemiological monitoring of APEC in poultry farms is essential.关键词
禽致病性大肠杆菌(APEC)/耐药基因/毒力基因/血清型/流行病学调查Key words
avian pathogenic Escherichia coli(APEC)/antimicrobial resistance gene/virulence gene/serotype/epidemio-logical investigation分类
农业科技引用本文复制引用
邵立志,吉星,孙利厂,朱树娇,王佳芸,何涛,王冉,杨章平..江苏省、山东省和安徽省禽致病性大肠杆菌的分子流行病学调查[J].中国兽医杂志,2025,61(2):1-9,9.基金项目
国家重点研发计划(2022YFD1800400) （2022YFD1800400）
