中国兽医杂志2025，Vol.61Issue(2)：40-46,7.DOI:10.20157/j.cnki.zgsyzz.2025.02.006
2株云南源BVDV-1b亚型毒株的分离和鉴定
Isolation and Identification of Two BVDV-1b Subtype Strains from Yunnan Province
摘要
Abstract
To investigate the occurrence,epidemiological trends,and biological characteristics of circulating strains of bovine viral diarrhea mucosal disease(BVD-MD)in Yunnan Province,this study randomly collected 667 bovine fecal samples from large-scale cattle farms across 10 prefectures in Yunnan between December 2022 and November 2023.The samples were tested for bovine viral diarrhea virus(BVDV)using reverse transcription-polymerase chain reaction(RT-PCR).Positive samples were used to isolate and identify strains on Madin-Darby bovine kidney(MDBK)cells,with virus propagation monitored through indirect immunofluorescence assay(IFA).Virus titers were determined using the Reed-Muench method.Whole-genome sequencing was performed on positive isolates,and phylogenetic analyses were conducted based on full-genome and 5′ untranslated region(5′-UTR)sequences.The results showed that two BVDV strains were successfully isolated and identified,named BVDV YN.1(GenBank accession No.:PP488512.1)and BVDV YN.2(GenBank accession No.:PP770551.1),with virus titers of 105.81 and 104.93 TCID50/mL,respectively.Phylogenetic analysis revealed that these two isolates shared 99.6%and 99.5%nucleotide similarity with the BVDV-1b subtype reference strain HP-KY-RK13(GenBank accession No.:KT355592.1)from the United States and 99.7%similarity with the BVDV-1b reference strain END(GenBank accession No.:JX419397.1)from Japan,clustering within the same evolutionary branch of the BVDV-1b subtype.This study provides theoretical insights for the epidemiological investigation and control of BVDV in Yunnan Province.关键词
牛病毒性腹泻病毒(BVDV)/BVDV-1b亚型/云南/分离和鉴定Key words
bovine viral diarrhea virus(BVDV)/BVDV-1b subtype/Yunnan Province/isolation and identification分类
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崔垚鑫,李聃,谢佳芮,朱沛,徐心明,岩锐,陈其云,李文贵,姚俊..2株云南源BVDV-1b亚型毒株的分离和鉴定[J].中国兽医杂志,2025,61(2):40-46,7.基金项目
云南省科技厅乡村振兴科技专项-科技特派团(202304BI090003) （202304BI090003）
云南省科技厅重点研发计划(202303AK140031) （202303AK140031）
云南益民养殖示范基地有限公司姚俊专家基层科研工作站 （）
澜沧重牧农业科技有限公司姚俊专家基层科研工作站 （）
