中国兽医杂志2025，Vol.61Issue(2)：47-51,5.DOI:10.20157/j.cnki.zgsyzz.2025.02.007
嗜水气单胞菌纳米PCR检测方法的建立
Establishment of a Nano-PCR Detection Method for Aeromonas hydrophila
摘要
Abstract
To rapidly detect Aeromonas hydrophila(A.hydrophila)infections in aquatic animals,this study designed primers based on the hlyA hemolysin gene sequence of A.hydrophila.A nano-polymerase chain reaction(nano-PCR)detection method was established by optimizing annealing temperature and primer volume.The sensitivity,specificity,and clinical sample detection results of the nano-PCR method were analyzed and compared with those of conventional PCR.The results showed that the optimized nano-PCR successfully amplified a specific A.hydrophila band of approximately 280 bp.The minimum detection concentration was 1.61 pg,and the sensitivity is 10 times higher than that of conventional PCR(with a minimum detection concentration of 16.1 pg).Both nano-PCR and conventional PCR showed no amplification for Vibrio vulnificus,Edwardsiella tarda,and Aeromonas veronii.The positive detection rate for 18 clinical samples was 72%,consistent with the results of conventional PCR.These findings indicate that the nano-PCR method developed in this study has higher sensitivity and specificity than conventional PCR,providing technical support for the differential diagnosis and control of A.hydrophila infections in aquatic animals.关键词
嗜水气单胞菌/纳米PCR/hlyA/临床检测Key words
Aeromonas hydrophila/nano-PCR/hlyA/clinical testing分类
农业科技引用本文复制引用
韩雅静,赵奇,石有权,安瑞,赵云萍,史秋梅,吴同垒,高桂生..嗜水气单胞菌纳米PCR检测方法的建立[J].中国兽医杂志,2025,61(2):47-51,5.基金项目
河北省科技厅循环养殖鱼类技术的应用与示范(202867012) （202867012）
河北省第三期现代农业产业技术体系技术河北省创新团队建设项目(HBCT2023220207) （HBCT2023220207）
