中国兽医杂志2025，Vol.61Issue(2)：57-61,5.DOI:10.20157/j.cnki.zgsyzz.2025.02.009
蒙古马马胃蝇三龄幼虫形态鉴定和种系发育关系分析
Morphological Identification and Phylogenetic Analysis of Third-Instar Larvae of Gasterophilus pecorum in Mongolian Horses
摘要
Abstract
To clarify the pathogenic species and phylogenetic relationships of equine gastric myiasis in Mongolian horses from Hohhot and Tongliao in the Inner Mongolia Autonomous Region,this study collected 120 third-instar larvae of Gasterophilus from slaughterhouses in March 2023.Morphological identification was performed using optical and scanning electron microscopy.The mitochondrial cytochrome oxidase subunit I(COX I)gene was amplified via PCR,and MEGA 6.0 and DnaSP 5.0 software were used to compare the COX I gene sequences with those of Gasterophilidae,Hypodermatidae,and Oestridae from GenBank.Phylogenetic trees were constructed,and haplotype and genetic differentiation analyses were conducted for larvae from Hohhot and Tongliao.The results showed that the third-instar larvae collected had pink body coloration and smooth oral cristae,consistent with the morphological features of Gasterophilus pecorum.Phylogenetic analysis revealed that the Tongliao isolates TONGLIAO1,TONGLIAO2,TONGLIAO3,and TONGLIAO4 clustered in the same branch as Hohhot isolate HUHEHAOTE2.Hohhot isolate HUHEHAOTE1 grouped with other Gasterophilidae species in GenBank.Both regions'Gasterophilus pecorum isolates formed separate branches from Gasterophilus nasalis,Gasterophilus haemorrhoidalis,and Gasterophilus intestinalis and were distant from Hypodermatidae,Oestridae,and the outgroup Diptera(Sarcophagidae).Sequence similarity analyses of the COX I gene corroborated the phylogenetic findings.Five haplotypes were identified among the mitochondrial COX I gene sequences of Gasterophilus pecorum from Hohhot and Tongliao,with no shared haplotypes.The fixation index(Fst)was greater than 0.05,and the gene flow(Nm)was 4.The results indicate that the pathogen of equine gastric myiasis in Hohhot and Tongliao is Gasterophilus pecorum,with close phylogenetic relationships and no genetic differentiation.关键词
蒙古马/黑腹胃蝇/形态学鉴定/发育树分析Key words
Mongolian horse/Gasterophilus pecorum/morphological identification/phylogenetic analysis分类
农业科技引用本文复制引用
董俊斌,包海泉..蒙古马马胃蝇三龄幼虫形态鉴定和种系发育关系分析[J].中国兽医杂志,2025,61(2):57-61,5.基金项目
国家自然科学基金(32360884) （32360884）
内蒙古自治区自然科学基金(2024MS03079) （2024MS03079）
