中国瓜菜2025，Vol.38Issue(2)：40-49,10.DOI:10.16861/j.cnki.zggc.2024.0634
番茄叶面积的遗传分析和BSA-QTL定位
Genetic analysis and BSA-QTL mapping of tomato leaf area
摘要
Abstract
Leaf area is one of the important agronomic traits in tomato that directly influences photosynthetic efficiency and material accumulation,thus significantly affecting plant growth and productivity.Studying the inheritance patterns of tomato leaf area and performing gene mapping through BSA-seq can provide a theoretical foundation for understanding the genetic and molecular mechanisms of leaf area in tomato.In this study,two parental lines with significant differences in leaf area,21GA04(P1)and 19GA13(P2),were selected to construct a population comprising six generations:P1,P2,F1,F2,BCP1 and BCP2.ImageJ software was used to measure the leaf area across these six generations.Genetic analysis of tomato leaf area was performed using a mixed genetic model combining major genes and polygenes.Additionally,bulked segregation analysis(BSA)was employed to assess the genotypes of extreme materials and parents,while QTL-seq(SNP-index association analysis)was used for preliminary localization of leaf area associated genes.The leaf area in the F2,BCP1 and BCP2 populations showed a skewed normal distribution.The most suitable genetic model for tomato leaf area is MX2-ADI-AD,indicating that this trait is controlled by two pairs of additive-dominant epistatic major genes and additive-dominant polygenes.SNP-index association analysis identified three QTL intervals,located on chromosome 1(89.0-89.8 Mb),chromosome 8(2.1-4.5 Mb),and chromosome 9(63.9-64.8 Mb).These results suggest that tomato leaf area is governed by both major genes and polygenes,with three associated regions located on chromosomes 1,8 and 9,and SlSAUR20 and SlSAUR21 are proposed as candidate genes associated with leaf area trait.关键词
番茄/叶面积/遗传分析/QTL定位Key words
Tomato/Leaf area/Genetic analysis/QTL mapping分类
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李珊珊,梁盼,孟繁艺,马梦秋,胡体旭,梁燕,蔡义勇,战祥强..番茄叶面积的遗传分析和BSA-QTL定位[J].中国瓜菜,2025,38(2):40-49,10.基金项目
宁夏回族自治区重点研发计划项目(2022BBF01001-02) （2022BBF01001-02）
陕西省重点研发计划项目(2021LLRH-07) （2021LLRH-07）
