中国瓜菜2025,Vol.38Issue(2):59-65,7.DOI:10.16861/j.cnki.zggc.2024.0477
可栽培黄色羊肚菌品种的鉴定与系统发育分析
Identification and phylogenetic analysis of cultivable yellow morel mush-rooms
摘要
Abstract
The ascocarps of yellow morel samples from a cultivation and domestication base were collected,and mycelial isolates were obtained through tissue separation.Conventional morphological characters combined with the analysis of concatenated sequences of internal transcribed spacers(ITS),elongation factor 1-alpha(EF1-α),RNA polymerase Ⅱsubunit 1/2(RPB1/RPB2)were used to classify and identify three cultivated yellow morel varieties.The macro-and micro-morphological characteristics of the ascocarps supported their identities of yellow morels.Phylogenetic analysis based on four gene fragments showed that the sequences of the three domesticated morel varieties clustered together with 10 morel reference sequences in the Esculenta clade,and with the Mes-21 in a monophyletic group(support rate 100%),indicating that they belong to the Mes-21 phylogenetic species..This is the first confirmed artificial cultivated species of Esculenta clade,which will effectively promote the development of yellow morel artificial cultivation technology and its large-scale cultivation applications in China.关键词
羊肚菌/支系/驯化栽培/系统发育学种/Mes-21Key words
Morel mushrooms/Esculenta clade/Domestication/Phylogenetic species/Mes-21分类
农业科技引用本文复制引用
张文昌,孙文化,王海彦,尹琪,孔维丽,何培新,刘伟..可栽培黄色羊肚菌品种的鉴定与系统发育分析[J].中国瓜菜,2025,38(2):59-65,7.基金项目
河南省现代农业产业技术体系(HARS-22-08-S,HARS-22-08-G1) (HARS-22-08-S,HARS-22-08-G1)