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可栽培黄色羊肚菌品种的鉴定与系统发育分析

张文昌 孙文化 王海彦 尹琪 孔维丽 何培新 刘伟

中国瓜菜2025,Vol.38Issue(2):59-65,7.
中国瓜菜2025,Vol.38Issue(2):59-65,7.DOI:10.16861/j.cnki.zggc.2024.0477

可栽培黄色羊肚菌品种的鉴定与系统发育分析

Identification and phylogenetic analysis of cultivable yellow morel mush-rooms

张文昌 1孙文化 2王海彦 3尹琪 1孔维丽 2何培新 1刘伟4

作者信息

  • 1. 郑州轻工业大学食品与生物工程学院 郑州 450001
  • 2. 河南省农业科学院食用菌研究所 郑州 450002
  • 3. 洛阳大荒野生态农业有限公司 河南洛阳 471000
  • 4. 中国科学院昆明植物研究所云南省真菌多样性与绿色发展重点实验室 昆明 650201
  • 折叠

摘要

Abstract

The ascocarps of yellow morel samples from a cultivation and domestication base were collected,and mycelial isolates were obtained through tissue separation.Conventional morphological characters combined with the analysis of concatenated sequences of internal transcribed spacers(ITS),elongation factor 1-alpha(EF1-α),RNA polymerase Ⅱsubunit 1/2(RPB1/RPB2)were used to classify and identify three cultivated yellow morel varieties.The macro-and micro-morphological characteristics of the ascocarps supported their identities of yellow morels.Phylogenetic analysis based on four gene fragments showed that the sequences of the three domesticated morel varieties clustered together with 10 morel reference sequences in the Esculenta clade,and with the Mes-21 in a monophyletic group(support rate 100%),indicating that they belong to the Mes-21 phylogenetic species..This is the first confirmed artificial cultivated species of Esculenta clade,which will effectively promote the development of yellow morel artificial cultivation technology and its large-scale cultivation applications in China.

关键词

羊肚菌/支系/驯化栽培/系统发育学种/Mes-21

Key words

Morel mushrooms/Esculenta clade/Domestication/Phylogenetic species/Mes-21

分类

农业科技

引用本文复制引用

张文昌,孙文化,王海彦,尹琪,孔维丽,何培新,刘伟..可栽培黄色羊肚菌品种的鉴定与系统发育分析[J].中国瓜菜,2025,38(2):59-65,7.

基金项目

河南省现代农业产业技术体系(HARS-22-08-S,HARS-22-08-G1) (HARS-22-08-S,HARS-22-08-G1)

中国瓜菜

OA北大核心

1673-2871

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