中国畜牧兽医2025，Vol.52Issue(2)：545-553,9.DOI:10.16431/j.cnki.1671-7236.2025.02.005
CRISPR-Cas技术在动物病原微生物检测中的应用研究进展
Advances in Application of CRISPR-Cas Technology in the Detection of Animal Pathogenic Microorganisms
摘要
Abstract
Animal infectious diseases are one of the most serious types of diseases that jeopardize the aquaculture industry,not only causing a large number of animal deaths and the loss of their products but also posing a major threat to human health.Therefore,rapid,accurate and inexpensive pathogen detection methods are of great significance for the prevention and control of animal infectious diseases.The clustered regularly interspaced short palindromic repeats(CRISPR)and associated proteins(Cas)system is a sensitive and specific biological system that can rapidly recognize pathogen-specific nucleic acid that plays an important role in the prokaryotic immune system.Pathogen detection methods based on the CRISPR-Cas system have the advantages of high specificity,high sensitivity and convenient operation,and have good prospects for application in the rapid detection of animal pathogenic microorganisms.The authors systematically introduced the type Ⅱ(cas9),type Ⅴ(cas12a,cas12b and cas14a)and type Ⅵ(cas13a)CRISPR-Cas systems,briefly described their working principles and application of NASBA-CRISPR,CAS-EXPAR,CASLFA,DETECTR,CORDS,HOLMESv2,SHERLOCK,SHERLOCKv2 and DETECTR-Cas14 established based on the CRISPR-Cas system in the detection of animal pathogenic microorganisms,and analyzed the problems faced by the CRISPR-Cas system in the detection of animal pathogens,including the impact of factors such as the specificity of the crRNA,the coupling with amplification technology,sample collection and transportation methods on quantitative detection,and proposed some solutions,aiming to provide a more in-depth understanding of the application of CRISPR-Cas technology in the detection of animal pathogenic microorganisms.关键词
病原微生物/CRISPR-Cas/病原体检测/应用Key words
pathogenic microorganisms/CRISPR-Cas/pathogen detection/application分类
农业科技引用本文复制引用
原青,孟茹,李国平,张瑞..CRISPR-Cas技术在动物病原微生物检测中的应用研究进展[J].中国畜牧兽医,2025,52(2):545-553,9.基金项目
国家自然科学基金项目(32302870) （32302870）
西南民族大学引进人才科研启动金资助项目(RQD2023034) （RQD2023034）
西南民族大学中央高校基本科研业务费专项基金项目(ZYN2023048) （ZYN2023048）
大通地区牦牛绿色防控技术集成与示范(2023-ZB-1) （2023-ZB-1）
