中国畜牧兽医2025，Vol.52Issue(2)：740-748,9.DOI:10.16431/j.cnki.1671-7236.2025.02.024
梅花鹿CSRP3基因克隆、生物信息学分析及其在肌肉组织中的表达分析
Cloning,Bioinformatics Analysis and Expression Analysis of CSRP3 Gene in Muscle Tissues of Sika Deer
摘要
Abstract
[Objective]The aim of this study was to investigate the biological function of cysteine and glycine-rich protein 3(CSRP3)gene in sika deer and its expression patterns in different muscle tissues.[Method]The longissimus dorsi muscle tissues of sika deer were used as experimental sample.The cDNA sequence of CSRP3 gene was cloned and sequenced,followed by sequence similarity alignment,construction of a phylogenetic tree,and bioinformatics analysis of CSRP3 protein.The expression differences of CSRP3 gene in longissimus dorsi muscle,gluteus maximus,intercostal muscle and quadriceps of sika deer was studied by Real-time quantitative PCR technology.[Result]The CSRP3 gene of sika deer was successfully cloned,and its coding region was 585 bp in size,encoding 194 amino acids.The BLAST comparison showed that CSRP3 gene of sika deer had the highest similarity with Cervus elaphus,at 99.83％,and the similarity with Odocoileus virginianus,Bos taurus,and Ovis aries were also over 95％,indicating that CSRP3 gene in sika deer had a high degree of conservation in evolution.The phylogenetic tree was constructed based on CSRP3 gene,and the results showed that sika deer was most closely related to that of Cervus elaphus and most distantly related to that of Panthera tigris and Orcinus orca.The molecular formula of CSRP3 gene-encoded protein was C903H1403N263O275S19,and the relative molecular mass was 20 952.81,the theoretical isoelectric point(pI)was 8.89,the fat coefficient was 43.81.The secondary structure of the protein was mainly random coil.Subcellular localization prediction indicated that the protein was located in the nucleus.Real-time quantitative PCR results showed that CSRP3 gene was expressed in different muscle tissues of sika deer.The expression level of this gene was the highest in the intercostal muscle of sika deer,with significant differences compared with the longissimus dorsi muscle,gluteus maximus,and quadriceps(P＜0.05),while the expression of CSRP3 gene in the longissimus dorsi muscle,gluteus maximus,and quadriceps were not significantly different(P＞0.05).[Conclusion]The CSRP3 gene of sika deer was successfully cloned in this study,which encoded a protein located in the nucleus of the cell.The CSRP3 gene was differentially expressed in different muscle tissues of sika deer.The results provided a data basis for investigating the regulatory mechanism of CSRP3 gene in sika deer meat quality.关键词
梅花鹿/CSRP3基因/克隆/肌肉/表达Key words
sika deer/CSRP3 gene/clone/muscle/expression分类
农业科技引用本文复制引用
王晴,马瑜锴,韩若冰,邢海华,孙子惠,李和平..梅花鹿CSRP3基因克隆、生物信息学分析及其在肌肉组织中的表达分析[J].中国畜牧兽医,2025,52(2):740-748,9.基金项目
黑龙江省学科协同创新成果建设项目(LJGXCG2022-008) （LJGXCG2022-008）
