中国药房2025，Vol.36Issue(4)：414-420,7.DOI:10.6039/j.issn.1001-0408.2025.04.05
蒙药三子散质量评价研究:指纹图谱、化学模式识别和多成分分析
Study on quality evaluation of Mongolian medicine Sanzi powder:fingerprint,chemical pattern recognition and multi-component quantification analysis
摘要
Abstract
OBJECTIVE To establish fingerprint,chemical pattern recognition and multi-component quantification analysis of Sanzi powder,and evaluate its quality.METHODS HPLC method was adopted.The fingerprints of 15 batches of Sanzi powder were established by using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 edition).Cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis were also conducted.The variable importance in projection(VIP)value greater than 1 was used as the index to screen the differential markers,and the contents of the differential markers were determined by the same HPLC method.RESULTS A total of 21 common peaks in the HPLC fingerprints of 15 batches of Sanzi powder were calibrated,and the similarities of them were 0.994-0.999;6 common peaks were identified,including gallic acid(peak 3),garminoside(peak 10),corilagin(peak 11),chebulinic acid(peak 16),ellagic acid(peak 18),crocin Ⅰ(peak 19).According to the results of cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis,15 batches of samples could be clustered into two categories:S1,S5,S7,S9,S14 were clustered into one category;S2-S4,S6,S8,S10-S13,S15 were clustered into one category.VIP values of 11 differential components such as corilagin,chebulinic acid and ellagic acid were higher than 1.Among 15 batches of samples,the contents of corilagin,chebulinic acid and ellagic acid ranged 2.667-5.152,9.506-13.522,0.891-1.811 mg/g.CONCLUSIONS Established HPLC fingerprint and multi-component quantification analysis of Sanzi powder are rapid and simple,and can be used for quality evaluation of Sanzi powder by combining with chemical pattern recognition.Eleven components such as corilagin,chebulinic acid and ellagic acid are differential markers affecting the quality of Sanzi powder.关键词
三子散/指纹图谱/化学模式识别/含量测定/质量评价Key words
Sanzi powder/fingerprint/chemical pattern recognition/content determination/quality evaluation分类
医药卫生引用本文复制引用
李君,李荣杰,周枫叶,张谦,张微,张博瀚,王舒,赵曦桐,陈建平..蒙药三子散质量评价研究:指纹图谱、化学模式识别和多成分分析[J].中国药房,2025,36(4):414-420,7.基金项目
国家自然科学基金项目(No.82460822) （No.82460822）
内蒙古自治区自然科学基金项目(No.2023QN08012,2024QN08054) （No.2023QN08012,2024QN08054）
内蒙古自治区卫生健康科技计划项目(No.202201187) （No.202201187）
内蒙古医科大学大学生创新创业项目(No.101322024017) （No.101322024017）
内蒙古医科大学联合项目(No.YKD2024LH005,No.YKD2023LH062) （No.YKD2024LH005,No.YKD2023LH062）
