中国药房2025，Vol.36Issue(4)：434-439,6.DOI:10.6039/j.issn.1001-0408.2025.04.08
落新妇苷对慢性肾功能衰竭大鼠肾损伤的影响及机制
Effects and mechanism of astilbin on renal injury in chronic renal failure rats
摘要
Abstract
OBJECTIVE To investigate the effect and potential mechanism of astilbin(AST)on renal injury in chronic renal failure(CRF)rats based on the Jagged-1/Notch-1 signaling pathway.METHODS CRF model was constructed by 5/6 nephrotomy.The successfully modeled rats were randomly separated into Model group,AST low-dose group(AST-L group),AST high-dose group(AST-H group),high-dose of AST+Notch pathway activator(Jagged-1/FC chimerin,referred to as"JFC")group(AST-H+JFC group),and control group(CK group)for open surgery without resection was set up,with 10 rats in each group.The rats in the AST-L group and AST-H group were given 40 and 80 mg/kg AST,respectively;the rats in the AST-H+JFC group were simultaneously given 80 mg/kg AST and 0.5 mg/kg JFC,and the rats in the CK group and Model group were given an equal volume of normal saline,once a day,for 4 weeks.After the last administration,the serum levels of blood urea nitrogen(BUN),serum creatinine(SCr),and the level of 24 h urinary protein(UP)in urine,as well as the serum levels of lactate dehydrogenase(LDH),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and IL-10 in each group were detected.The morphology and fibrosis of renal tissue were observed.The content of adenosine triphosphate(ATP)and the activities of sodium-potassium ATPase and calcium-magnesium ATPase in mitochondria of renal tissue were detected.The protein expressions of transforming growth factor-β(TGF-β),hypoxia-inducible factor-1α(HIF-1α),α-smooth muscle actin(α-SMA),cleaved-caspase-3,Jagged-1 and Notch-1 in renal tissue were also observed.RESULTS Compared with CK group,the renal tissue of rats in the Model group was obviously damaged,renal tissue fibrosis was severe;the serum BUN and SCr levels,urine UP level,serum LDH,TNF-α and IL-6 levels,as well as the protein expressions of TGF-β,HIF-1α,α-SMA,cleaved-caspase-3,Jagged-1 and Notch-1 in renal tissue were significantly increased,while the serum IL-10 level,ATP content and activities of sodium-potassium ATPase and calcium-magnesium ATPase in mitochondria of renal tissue were significantly decreased(P＜0.05).Compared with Model group,the renal tissue damage and fibrosis in the AST groups were reduced,the serum BUN and SCr levels,urine UP level,serum LDH,TNF-α and IL-6 levels,and the protein expressions of TGF-β,HIF-1α,α-SMA,cleaved-caspase-3,Jagged-1 and Notch-1 were significantly decreased,while the serum IL-10 level,ATP content and the activities of sodium-potassium ATPase and calcium-magnesium ATPase in mitochondria were significantly increased;the changes in the aforementioned indicators in AST-H group were more significant than those in the AST-L group(P＜0.05).JFC could significantly reverse the improvement effect of high dose of AST on renal injury in CRF rats(P＜0.05).CONCLUSIONS AST can reduce inflammation in CRF rats,alleviate renal tissue damage and fibrosis,and improve renal mitochondrial capacity metabolism,possibly by inhibiting the Jagged-1/Notch-1 signaling pathway.关键词
落新妇苷/慢性肾功能衰竭/肾损伤/肾纤维化/Jagged-1/Notch-1信号通路Key words
astilbin/chronic renal failure/renal injury/renal fibrosis/Jagged-1/Notch-1 signaling pathway分类
医药卫生引用本文复制引用
高晓卫,刘迎迎,韩聪,郝世飞..落新妇苷对慢性肾功能衰竭大鼠肾损伤的影响及机制[J].中国药房,2025,36(4):434-439,6.基金项目
山东省医药卫生科技发展计划项目(No.2019WS578) （No.2019WS578）
