种子2025，Vol.44Issue(1)：10-18,30,10.DOI:10.16590/j.cnki.1001-4705.2025.01.010
基于SRAP和ISSR标记的花椒种质资源遗传多样性及群体结构分析
Genetic Diversity and Population Structure Analysis of Zanthoxylum bungeanum Germplasm Resources Based on SRAP and ISSR Markers
摘要
Abstract
In order to explore the genetic diversity and population structure of Zanthoxylum bungea-num germplasm resources in China,the genetic diversity,genetic relationship and population struc-ture of 48 Zanthoxylum bungeanum germplasm resources were analyzed by ISSR and SRAP moecular marker technology.The results showed that 43 polymorphic bands and 56 polymorphic bands were amplified by 6 pairs of SRAP and 7 ISSR primers,respectively,and the polymorphism ratio was 87.73％and 88.89％.The mean Nei's diversity index and the mean Shannon's information index were 0.437 2 and 0.621 7 respectively.The genetic diversity of 8 Zanthoxylum bungeanum populations was analyzed according to different origins,including of Zunyi population Guizhou province＞Shaanxi pop-ulation＞Sichuan population＞Qianxinan population of Guizhou＞Jiangxi population＞Tongren popu-lation of Guizhou＞Qiandongnanzhou population of Guizhou＞Guiyang population of Guizhao.Molec-ular genetic variation analysis showed that 92％of the genetic variation of Zanthoxylum bungeanum germplasm resources occurred within provenances,and only 8％occurred between provenances.The results of population genetic Structure and UPGMA clustering showed that there was a moderate de-gree of gene exchange among provenances.The polymorphism of Zanthoxylum bungeanum germ-plasm resources could be detected efficiently by SRAP and ISSR markers.The genetic differentiation of Zanthoxylum bungeanum germplasm resources was obvious among species,while the genetic dif-ferentiation of different sources of the same species was small.关键词
花椒/SRAP分子标记/ISSR分子标记/遗传多样性/群体结构分析Key words
Zanthoxylum bungeanum/SRAP molecular markers/ISSR molecular markers/genetic diversity/population structure analysis分类
农业科技引用本文复制引用
李晋华,汪志燚,王少铭,冷家归,侯颖辉,罗莉斯,李德文..基于SRAP和ISSR标记的花椒种质资源遗传多样性及群体结构分析[J].种子,2025,44(1):10-18,30,10.基金项目
国家重点研发计划项目(2022YFD1601700) （2022YFD1601700）
国家重点研发计划课题(2022YFD1601711) （2022YFD1601711）
贵州省农业科学院青年基金项目(黔农科一般基金[2025]46) （黔农科一般基金[2025]46）
