转化医学杂志2024，Vol.13Issue(10)：1651-1655,5.DOI:10.3639/i.issn.2095-3097.2024.10.021
耐多药肺结核患者外周血Toll样受体2 mRNA表达与Th1/Th2/Th17细胞因子变化及预后关系
Correlation between the Expression of Toll-like Receptor 2 mRNA in Peripheral Blood and the Changes of Th1/Th2/Th17 Cytokines and Prognosis in Patients with Multidrug-Resistant Tuberculosis
摘要
Abstract
Objective To investigate the relationship between the peripheral blood Toll-like receptor 2(TLR2)mRNA expression,T helper cell(Th)1/Th2/Th17 cytokine changes,and prognosis in multidrug-resistant tuberculosis(MDR-TB)patients.Methods A total of 30 MDR-TB patients,30 drug-susceptible tuberculosis(DS-TB)patients,and 30 healthy individuals with positive tuberculin pure protein derivative(PPD+)test results were included from November 2021 to May 2023.TLR2 mRNA levels in peripheral blood were detected by fluorescence quantitative PCR.Flow cytometry was used to measure CD4 cell ratio,T-bet+CD4+cell ratio,interleukin-4(IL-4),IL-17,and interferon-γ(IFN-γ)levels.Multivariate Logistic regression analysis was conducted to identify factors influencing MDR-TB prognosis.Results The CD4 cell ratio and IFN-γ levels in the PPD+group were significantly higher than those in the DS-TB and MDR-TB groups,while the IL-4,IL-17,and TLR2 mRNA levels were significantly lower(P＜0.05).In MDR-TB patients,the CD4 cell ratio and IFN-γ levels in the good prognosis group were significantly higher than those in the poor prognosis group,while IL-4,IL-17,and TLR2 mRNA levels were significantly lower(P＜0.05).Increased CD4 cell ratio and IFN-γ levels were protective factors,while elevated IL-4,IL-17,and TLR2 mRNA levels were risk factors for poor prognosis(P＜0.05).Conclusion High expression levels of TLR2 mRNA,IL-4,and IL-17 in peripheral blood are risk factors for poor prognosis in MDR-TB patients,while high CD4 cell ratio and IFN-γ levels serve as protective factors.Monitoring these biomarkers may have clinical significance in evaluating the prognosis of MDR-TB.关键词
肺结核/耐药性肺结核/Toll样受体2/辅助性T细胞1/辅助性T细胞17/细胞因子Key words
Tuberculosis/Drug-resistant tuberculosis/Toll-like receptor 2/T helper cell 1/T helper cell 17/Cytokine分类
医药卫生引用本文复制引用
程小会,唐铮,李欣芹,李飞,李洁,余雷,林青..耐多药肺结核患者外周血Toll样受体2 mRNA表达与Th1/Th2/Th17细胞因子变化及预后关系[J].转化医学杂志,2024,13(10):1651-1655,5.基金项目
重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0715) （cstc2020jcyj-msxmX0715）
重庆市九龙坡区公共卫生重点学科和实验室建设经费资助(JLPWBF202363) （JLPWBF202363）
