杂交水稻2025，Vol.40Issue(1)：27-36,10.DOI:10.16267/j.cnki.1005-3956.20240401.102
苯达松敏感型智能雌性不育恢复系的创制
Creation of Bentazon-sensitive Genetic Engineering Female Sterile Restorer Lines
摘要
Abstract
The increasing cost of seed production restricts the sustainable development of hybrid rice.The mechanized seed production technology of female sterility is a kind of mixed sowing and mixed harvest seed production technology based on female sterile restorer lines,but this technology faces the problems of a long cycle of female sterile parent creation and incomplete female abortion of restorer lines.The female fertility related gene PTB1,bentazon resistance gene Bel and fragrance gene Badh2 in rice restorer lines were knocked out by gene editing technology,and the gene elements of genetic engineering female sterile restorer lines were introduced into the homozygous mutants obtained through gene editing to realize the creation of bentazon-sensitive genetic engineering female sterile restorer lines.It solves the problems of a long cycle of parent creation and incomplete female sterility in mechanized seed production of female sterility,promotes the practical application of mechanized seed production technology of female sterility,and is of great significance to the development of hybrid rice seed production technology.关键词
杂交水稻/基因编辑技术/苯达松敏感/智能雌性不育恢复系/混播混收/机械化制种Key words
hybrid rice/gene editing technology/bentazon sensitivity/genetic engineering female sterile restorer line/mixed sowing and mixed harvest/mechanized seed production分类
农业科技引用本文复制引用
唐宁,夏玉梅,詹祎捷,淡俊豪,王耀,田钧友,陈劲,盛夏冰,曹孟良..苯达松敏感型智能雌性不育恢复系的创制[J].杂交水稻,2025,40(1):27-36,10.基金项目
湖南省农业科技创新资金(2022CX114,2022CX84-05) （2022CX114,2022CX84-05）
杂交水稻全国重点实验室自主研究项目(2022QN04) （2022QN04）
湖南省自然科学基金青年基金(2022JJ40295) （2022JJ40295）
国家自然科学基金青年基金(32301765) （32301765）
湖南省自然科学基金青年基金(2022JJ40296) （2022JJ40296）
