杂交水稻2025，Vol.40Issue(1)：37-43,7.DOI:10.16267/j.cnki.1005-3956.20240306.073
多突变类型低镉香型抗稻瘟病水稻新种质的创制
Creation of Various Mutation Types of New Rice Germplasms with Low Cadmium Accumulation,Aroma and Blast Resistance
摘要
Abstract
The OsNramp5 gene controls the uptake and translocation of cadmium(Cd)in rice,and plays an important role in the breeding of rice varieties with low cadmium accumulation.The 9th,10th and 13th exons of the OsNramp5 gene were knocked out by CRISPR/Cas9 gene editing technology with the aromatic blast-resistant indica rice material N5 as the receptor.The nucleobase deletion mutants N9-1 and N9-2 and nucleobase insertion mutant N9-3 in exon 9,the nucleobase insertion mutant N10-1 in exon 10 and the nucleobase deletion mutant N13-1 in exon 13 were created without exogenous gene components.In the experimental field with soil total cadmium content of 1.00±0.02 mg/kg and pH 5.6,the cadmium content in brown rice of each mutant decreased by 97.60％～98.08％compared with the wild type,which was significantly lower than the national standard brown rice limit index of 0.20 mg/kg,and there was no significant difference between the mutants.The main agronomic traits such as plant height,productive panicles per plant,1 000-grain weight and grain yield of each mutant were not significantly different from those of wild type.Various mutation types of new indica rice materials with low cadmium accumulation,aroma and blast resistance were created,which provided technical support and material basis for the breeding of new low-cadmium rice varieties.关键词
水稻/低镉/CRISPR/Cas9技术/基因编辑/OsNramp5基因/突变体/新种质Key words
rice/low cadmium accumulation/CRISPR/Cas9 technology/gene editing/OsNramp5 gene/mutant/new germplasm分类
农业科技引用本文复制引用
程紫钦,张家清,赵正洪,张海清,胡文彬,刘腾飞,周政,刘烨,孙海东,文萱,贺永慧,贺记外..多突变类型低镉香型抗稻瘟病水稻新种质的创制[J].杂交水稻,2025,40(1):37-43,7.基金项目
湖南省农业科技创新资金项目(2022CX21,2023CX87) （2022CX21,2023CX87）
