作物学报2025，Vol.51Issue(3)：586-597,12.DOI:10.3724/SP.J.1006.2025.44112
谷子SHMT基因家族全基因组鉴定与表达分析
Genome-wide identification and expression analysis of SHMT gene family in foxtail millet(Setaria italica L.)
摘要
Abstract
Serine hydroxymethyltransferase(SHMT)is involved in carbon metabolism and photorespiration,and is widely pre-sent in crops,playing a critical role in growth,development,and stress resistance.However,the SHMT genes in foxtail millet are largely unexplored.In this study,we identified the members of the SiSHMT gene family at the whole-genome level and system-atically analyzed their gene structures,evolutionary relationships,chromosomal localizations,interspecies collinearity,cis-acting elements,expression patterns,and dominant haplotypes.Our results revealed five SiSHMT members in foxtail millet,with mo-lecular weights ranging from 51.70 to 64.37 kD,and similar spatial structures.Phylogenetic analysis classified these genes into three groups,with members distributed across different chromosomes.The analysis of cis-acting elements in the gene promoters indicated the presence of numerous photo-responsive elements,anaerobic response elements,hormone response elements,and other cis-acting elements.Interspecies collinearity analysis showed that SiSHMT3 and SiSHMT4 exhibited collinearity with their orthologous genes in monocot crops such as rice,wheat,sorghum,and maize,with SiSHMT3 displaying multiple collinear pairs with rice,wheat,and maize.The expression levels of SiSHMT family members varied across different developmental stages and tissues of foxtail millet.Notably,SiSHMT4 was highly expressed in developing panicles and was significantly induced by drought,salt,and ABA treatments.Haplotype analysis of SiSHMT4 revealed that Hap1 was the dominant haplotype,significantly outper-forming other haplotypes in panicle length,width,and weight.These findings provide valuable gene resources for improving drought and salt tolerance in foxtail millet and lay a theoretical foundation for the breeding of high-yield,stress-resistant foxtail millet varieties in the future.关键词
谷子/SHMTs/基因家族/生长发育/非生物胁迫Key words
foxtail millet/SHMTs/gene family/growth and development/abiotic stress引用本文复制引用
郭冰,秦家范,李娜,宋梦瑶,王黎明,李君霞,马小倩..谷子SHMT基因家族全基因组鉴定与表达分析[J].作物学报,2025,51(3):586-597,12.基金项目
本研究由河南省重点研发专项(231111110300),国家自然科学基金项目(32301841),河南省自然科学基金项目(242300421320)和河南科技大学A类博士人才项目(13480103)资助.This study was supported by the Henan Provincial Key Research and Development Project(231111110300),the National Natural Science Foundation of China(32301841),the National Natural Science Foundation of Henan Province(242300421320),and the Category A PhD Research Startup Foundation of Henan University of Science and Technology(13480103). （231111110300）
