作物学报2025，Vol.51Issue(3)：598-608,11.DOI:10.3724/SP.J.1006.2025.42028
水稻MKKs家族基因成员OsMKK4的不同等位基因型及自然变异对籽粒的影响
Effects of different alleles and natural variations of OsMKK4,a member of the rice MKKs family gene,on grains
摘要
Abstract
The MKK gene family in the MAPK signaling pathway plays a pivotal role in connecting processes related to rice growth,development,and defense signaling,regulating various biological processes.In this study,we identified MKK genes in rice through bioinformatics analysis and constructed a phylogenetic tree,revealing both the conservation and diversity within the MKK gene family.Using gene editing technology,we edited the OsMKK4 gene in the Nipponbare rice variety and successfully obtained six lines with different mutation types in this gene.Grain length,grain width,and 1000-grain weight in these mutant lines were significantly reduced compared to the wild type.Haplotype analysis revealed clear differentiation in OsMKK4 gene haplotypes between indica and japonica rice,primarily divided into the Glu14 japonica type and Leu14 indica type.On average,japonica rice containing Glu14 exhibited shorter grain length but higher grain width and 1000-grain weight.Furthermore,intro-ducing the OsMKK4 gene with the Leu14 haplotype from Kasalath into Nipponbare resulted in significantly reduced grain length,grain width,and 1000-grain weight in the DHX(CSSL)compared to Nipponbare.However,compared to Kasalath,the grain width and 1000-grain weight of the substitution line increased significantly,particularly in grain width.This study highlights the influence of key OsMKK4 gene haplotypes on rice grain traits,providing valuable genetic resources and strategies for more precise molecular breeding design.关键词
水稻/MAPK/OsMKK4/粒型/单倍型Key words
rice/MAPK/OsMKK4/grain size/haplotype引用本文复制引用
刘建国,陈冬东,陈玉玉,易琴琴,李清,徐正进,钱前,沈兰..水稻MKKs家族基因成员OsMKK4的不同等位基因型及自然变异对籽粒的影响[J].作物学报,2025,51(3):598-608,11.基金项目
本研究由浙江省自然科学基金探索项目(LY22C130005)和国家自然科学基金项目(32171987)资助.This study was supported by the Zhejiang Provincial Natural Science Foundation Exploration Project(LY22C130005)and the National Natural Science Foundation of China(32171987). （LY22C130005）
