作物学报2025，Vol.51Issue(3)：609-620,12.DOI:10.3724/SP.J.1006.2025.41021
甘肃省小麦农家种老芒麦分子鉴定及其重要性状评价
Molecular characterization and evaluation of important traits of landrace wheat Laomangmai in Gansu province,China
摘要
Abstract
This study aimed to clarify the heterogeneity of ten Laomangmai landrace wheat varieties from Gansu,which share the same name but originate from different regions.Agronomically important traits and disease resistance were evaluated in the field,and AFLP markers were used to create genetic fingerprints.Alleles at four vernalization loci,one photoperiod locus,four dwarf-ing loci,three multi-resistance loci,and key alleles related to gluten strength and pigment content were detected.The results were as follows:(1)The genetic similarity among the ten Laomangmai landraces was very low,indicating high heterogeneity.(2)In terms of yield-related traits,all materials carried recessive alleles at the vernalization loci Vrn-A1,Vrn-B1,and Vrn-B3.Seven landraces,including six Laomangmai varieties and the check variety Juanmangheshangtou,had the dominant allele at Vrn-D1,resulting in significantly earlier heading after spring sowing,but no effect on heading date under autumn sowing conditions.Only one variety(code 5)contained the photoperiod-insensitive allele Ppd-D1a.Of the four dwarfing genes studied,five landraces carried the dwarfing allele at the Rht-24 locus,while the remaining landraces exhibited high allelic variation.The Rht-24 dwarfing allele did not significantly reduce plant height but was associated with a higher number of grains per spike compared to the tall genotypes.(3)All materials showed poor lodging resistance.Four Laomangmai landraces exhibited strong cold tolerance,includ-ing three winter and one spring growth habit varieties.Three Laomangmai landraces(codes 1,4,and 10)were highly resistant to stripe rust,despite not carrying known stripe rust resistance genes.Additionally,three other landraces,including two Laomangmai varieties(codes 7 and 9)and the check variety,contained the pleiotropic resistance gene Yr18/Lr34/Sr57/Pm38.(4)The gluten strength of the studied landraces was weak.Only four varieties(codes 5,6,7,and 8)carried low PPO-activity alleles,while all materials carried alleles for high yellow pigment content and high peroxidase activity.(5)The varieties carrying the dominant allele at Vrn-D1 could be useful for spring wheat cultivation in central and western Gansu Province,the upstream Jialing River winter wheat region,and similar ecological areas.The five varieties carrying the dwarfing allele Rht-24b could be employed for breeding fusarium head blight resistance in Gansu Longnan,and the middle and lower Yangtze River valley.Landraces carrying Yr18/Lr34/Sr57/Pm38,with high resistance to stripe rust,hold potential for breeding disease-resistant varieties in Longnan,Tianshui,Longdong,and other stripe rust hot spots.Additionally,the four varieties with alleles for low polyphenol oxidase acti-vity could be useful for improving pigment content in wheat breeding.This study clarified the genetic characteristics,strengths,and weaknesses of important traits in the Laomangmai varieties from different regions in Gansu province,providing guidance for their application in wheat breeding programs.关键词
小麦/地方种/老芒麦/AFLP/分子标记检测/异质性/性状评价Key words
common wheat/landrace/Laomangmai/AFLP/molecular marker detection/heterogeneity/character evaluation引用本文复制引用
展宗冰,靳奇峰,刘迪,吕迎春,郭莹,张雪婷,虎梦霞,王尚,杨芳萍..甘肃省小麦农家种老芒麦分子鉴定及其重要性状评价[J].作物学报,2025,51(3):609-620,12.基金项目
本研究由甘肃省农业科学院区域协同创新项目(2024GAAS05),甘肃省科技计划项目-重点研发计划(23YFNA0033)和特派团专项(22CX8NA027)和国家自然科学基金项目(32060481,32260485)资助.This study was supported by the Regional Collaborative Innovation Project(2024GAAS05)of Gansu Academy of Agricultural Sciences,the Key Research and Development Plan(23YFNA0033),the Special Mission Project(22CX8NA027)of Gansu Province Science and Tech-nology Plan Project,and the National Natural Science Foundation of China(32060481,32260485). （2024GAAS05）
