作物学报2025，Vol.51Issue(3)：621-631,11.DOI:10.3724/SP.J.1006.2025.44120
芥菜型油菜响应菌核病侵染表达特性与高抗性关联基因分析
Analysis of genes associated with expression characteristics and high resistance in response to Sclerotinia sclerotiorum infection in Brassica juncea
摘要
Abstract
Sclerotinia sclerotiorum is a major disease affecting rapeseed,often causing significant yield losses after infection.In this study,we evaluated the resistance of 200 Brassica juncea lines to S.sclerotiorum and selected one highly resistant line(G21-243-1,HR)and one susceptible line(G21-149-2,LR)for transcriptome analysis.A total of 138.16 Gb of clean data was generated by simulating leaf responses at 12,24,and 36 hours post-infection(hpi)with S.sclerotiorum.The analysis identified 1899 up-regulated genes and 1330 down-regulated genes,with 445 differentially expressed genes(DEGs)detected across two time points and 90 DEGs across all three time points.Subsequent Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional analyses revealed significant enrichment in pathways related to plant-pathogen interactions,plant hormone signaling,and the MAPK signaling pathway in plants.By integrating transcriptome and functional analysis results,we preliminarily identified 20 candidate genes associated with resistance to S.sclerotiorum.Six of these genes were randomly selected for validation via qRT-PCR analysis.The findings of this study provide a foundation for future research on S.scle-rotiorum,including the characterization of gene expression,the identification of resistance genes,and the development of resistant rapeseed varieties.关键词
芥菜型油菜/菌核病/转录组分析/差异表达基因Key words
Brassica juncea/Sclerotinia sclerotiorum/transcriptome analysis/differentially expressed genes引用本文复制引用
张金泽,周庆国,杨旭,王倩,肖莉晶,金海润,欧阳青静,余坤江,田恩堂..芥菜型油菜响应菌核病侵染表达特性与高抗性关联基因分析[J].作物学报,2025,51(3):621-631,11.基金项目
本研究由贵州省科技支撑计划项目(黔科合支撑[2022]重点031),国家自然科学基金项目(32160483,32360497),贵州大学国家自然科学基金项目([2023]093号),贵州省粮油作物分子育种重点实验室项目(黔科合中引地[2023]008)和贵州省高等学校功能农业重点实验室项目(黔教技[2023]007号)资助.This study was supported by the Guizhou Provincial Science and Technology Support Program(Guizhou Kehe Support[2022]Key 031),the National Natural Science Foundation of China(32160483,32360497),the National Natural Science Foundation of Guizhou University([2023]No.093),the Guizhou Provincial Key Laboratory of Molecular Breeding of Grain and Oil Crops(Guizhou Kehezhong Yindi[2023]008),and the Guizhou Provincial Key Laboratory of Functional Agriculture in Colleges and Universities(Guizhou Jiaoji[2023]No.007). （黔科合支撑[2022]重点031）
