作物学报2025，Vol.51Issue(3)：632-649,18.DOI:10.3724/SP.J.1006.2025.41033
小麦TaSnRK基因家族鉴定及在局部根区干旱下的表达分析
Identification of TaSnRK gene family and expression analysis under localized root zone drought in wheat
摘要
Abstract
Sucrose non-fermenting-1-related protein kinase(SnRK)plays a critical regulatory role in response to abiotic stress.To systematically analyze the physicochemical properties,chromosome distribution,gene structure,phylogenetic relationships,and expression characteristics of the TaSnRK gene family in wheat(Triticum aestivum L.)under local root zone drought stress,this study employed bioinformatics approaches to identify the full complement of TaSnRK genes in the wheat genome.Their expres-sion patterns under local root zone drought stress were analyzed using a wheat expression database and quantitative real-time PCR(qRT-PCR).The analysis identified 139 members of the SnRK gene family in wheat,which were categorized into three subfami-lies:15 members in SnRK1,31 in SnRK2,and 93 in SnRK3.Protein sequence lengths ranged from 154 to 836 amino acids.Con-served motif analysis revealed that all members of the three subfamilies shared Motif2 and Motif4.Additionally,all SnRK1 members contained Motif14 and Motif15,which were absent in SnRK2 and SnRK3 subfamilies.In contrast,all SnRK3 members contained Motif10,which was not found in SnRK1 and SnRK2 subfamilies.Intraspecific collinearity analysis indicated that the TaSnRK genes had a total of 217 duplication events,showing high homology and strong conservation during evolution.The Ka/Ks ratio suggested that only four pairs of TaSnRK genes were under positive selection pressure.Cis-regulatory element analysis re-vealed that most of the cis-elements in the TaSnRK genes were associated with growth and development,as well as various stress-responsive elements.Gene expression pattern analysis showed that only 20 TaSnRK genes exhibited relatively high expres-sion levels in grains,whereas 85,90,92,and 80 genes were highly expressed in panicles,leaves,buds,and roots,respectively.qRT-PCR analysis confirmed that TaSnRK expression was higher in drought-resistant wheat varieties,with SnRK2 and SnRK3 subfamily members playing key roles in sensing and transmitting drought stress signals.Protein-protein interaction analysis iden-tified 267 interaction events between 35 TaSnRK proteins and 23 related functional proteins.These findings provide a theoretical foundation for further understanding the role of TaSnRK genes in regulating wheat growth,development,and drought stress responses.关键词
小麦/TaSnRK基因/生物信息学/局部根区干旱/基因表达Key words
wheat/TaSnRK gene/bioinformatics/local root zone drought/gene expression引用本文复制引用
张恒,冯雅岚,田文仲,郭彬彬,张均,马超..小麦TaSnRK基因家族鉴定及在局部根区干旱下的表达分析[J].作物学报,2025,51(3):632-649,18.基金项目
本研究由国家自然科学基金项目(32372227),河南省高等学校青年骨干教师培养计划(2021GGJS050)和河南省科技研发计划联合基金项目(232103810021)资助.This study was supported by the National Natural Science Foundation of China(32372227),the Training Program for University Young Key Teachers in Henan Province(2021GGJS050),and the Henan Provincial Science and Technology Research and Development Plan Joint Fund(232103810021). （32372227）
