作物学报2025，Vol.51Issue(3)：676-686,11.DOI:10.3724/SP.J.1006.2025.42031
铝胁迫下水稻osalr3突变体对外源有机酸和植物生长调节物质的响应
Response of osalr3 mutant to exogenous organic acids and plant growth regulators under aluminum stress
摘要
Abstract
Soil acidification and aluminum(Al)toxicity have become significant challenges affecting rice growth and yield.Therefore,it is crucial to investigate Al-tolerance-related genes and elucidate their molecular mechanisms.In a previous Ge-nome-Wide Association Study(GWAS),the gene OsAlR3,associated with Al tolerance,was identified.OsAlR3 was shown to regulate Al tolerance in rice through phenotypic and physiological mechanisms.While organic acids and plant growth regulators are known to be involved in Al resistance,the specific role of OsAlR3 in these processes remains unclear.This study examined the responses of wild-type(WT)and osalr3 knockout mutants to exogenous organic acids(citric acid(CA),oxalic acid(OA),and malic acid(MA))and plant growth regulators(brassinolide(BR)and auxin(IAA)).Compared to WT,the osalr3 mutant exhibited significantly reduced total root length,inter-root pH,and organic acid content,while above-ground and below-ground Al3+content,malondialdehyde(MDA),superoxide anion(O2-),hydrogen peroxide(H2O2)content,and superoxide dismutase(SOD)activity were significantly increased under Al stress.The osalr3 mutant showed heightened sensitivity to Al stress compared to WT.Exogenous application of organic acids and BR increased total root length,CA,OA,and MA content,and decreased above-ground and below-ground Al3+content,MDA,O2-,H2O2 content,and SOD activity,thereby reducing the Al toxicity in osalr3 mutants.In contrast,although auxin application increased total root length and reduced above-ground Al3+content in the mutant,it significantly increased below-ground Al3+content,along with MDA,O2-,and H2O2 levels,which were markedly different from those of WT.In summary,exogenous application of organic acids(CA,OA,and MA)and BR positively influ-enced OsAlR3 function,while IAA application negatively regulated OsAlR3 function under Al stress.关键词
水稻/OsAlR3/有机酸/植物生长调节物质/铝毒害Key words
rice/OsAlR3/organic acids/plant growth regulators/Al toxicity引用本文复制引用
苏畅,满福原,王镜博,冯晶,姜思旭,赵明辉..铝胁迫下水稻osalr3突变体对外源有机酸和植物生长调节物质的响应[J].作物学报,2025,51(3):676-686,11.基金项目
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-01-09),辽宁省科技重大专项(2022JH1/10200003)和兴辽英才项目(XLYC2008025)资助.This study was supported by the China Agriculture Research System of MOF and MARA(CARS-01-09),the Major Scientific and Techno-logical Special Project of Liaoning Province(2022JH1/10200003),and the Liaoning Revitalization Talents Program(XLYC2008025). （CARS-01-09）
