植物遗传资源学报2025，Vol.26Issue(2)：260-270,11.DOI:10.13430/j.cnki.jpgr.20240412001
基于SCoT标记的黑稻种质资源遗传多样性分析
Genetic Diversity Analysis of Black Rice Germplasm Resources Based on SCoT Markers
摘要
Abstract
Considering the complexity of black rice germplasm resources on distribution and origin,uncovering the genetic diversity of black rice materials and clarify their genetic relationship becomes of significance.This study analyzed the genetic diversity of 90 rice materials(including 21 black leaf black rice,24 purple leaf black rice,30 green leaf black rice,and 15 white rice)by using 30 SCoT primers.A total of 194 fragments were detected by SCoT primers,including 165 that showed polymorphic with the ratio of 85％.The average values of allele number(Na),effective allele number(Ne),Nei's gene diversity index(H)and Shannon's information index(Ⅰ)were 1.9216,1.3922,0.2445 and 0.3836,respectively.The results of cluster analysis showed that at a genetic similarity coefficient of 0.73,these genotypes were clustered into four groups,coincidence with the results of population structure analysis.The genetic similarity coefficient predominantly ranged from 0.59 to 0.79,indicating a relatively high genetic similarity.Analysis of molecular variance(ANOVA)showed that 91％of the genetic variations were within the population,with only 9％being between the populations.These findings indicate that there is limited genetic diversity in the collection,and enriching genetic diversity of black rice germplasm resources required further efforts.This study provides valuable insights for parental selection in black rice hybridization,and offers a theoretical basis for genetic improvement and cultivation of new black rice varieties.关键词
黑稻/SCoT标记/遗传多样性Key words
black rice/SCoT marker/genetic diversity引用本文复制引用
刘柯忻,张晓娟,张卫平,李新生,吴升华,马秀奇..基于SCoT标记的黑稻种质资源遗传多样性分析[J].植物遗传资源学报,2025,26(2):260-270,11.基金项目
陕西省科技厅项目(2023-YBNY-267) （2023-YBNY-267）
秦巴生物资源与生态环境省部共建国家重点实验室(培育)"市校共建"科研专项项目(SXC-2303)Shaanxi Provincial Department of Science and Technology Project(2023-YBNY-267) （培育）
The Ministry of Biological Resources and Ecology and Environment of Qinba Jointly Established the State Key Laboratory(Cultivation)of the"City-School Co-construction"Scientific Research Project(SXC-2303) （Cultivation）
