植物遗传资源学报2025，Vol.26Issue(2)：271-283,13.DOI:10.13430/j.cnki.jpgr.20240527001
基于表型性状和SSR标记的知母遗传多样性及关联分析
Genetic Diversity and Correlation Analysis of Anemarrhena asphodeloides Based on Phenotypic Traits and SSR Markers
摘要
Abstract
In order to clarify the genetic background of the germplasm of Anemarrhena asphodeloides,the genetic diversity of 160 germplasm materials of A.asphodeloides was analyzed based on phenotypic traits and SSR markers using cluster analysis and principal component analysis.The results showed that the diversity index of 21 phenotypic traits ranged from 0.99 to 7.60,with an average value of 1.99.The coefficient of variation ranged from 17.22％to 151.07％,with an average of 51.49％.A total of 143 alleles were detected by capillary electrophoresis using 27 pairs of newly developed SSR primers.The effective alleles ranged from 0.56 to 7.40,with an average of 3.54.Shannon's information index ranges from 0.17 to 0.51,with an average value of 0.36.Polymorphism information content(PIC)ranged from 0.15 to 0.50,with an average of 0.38.Cluster analysis showed that there was no obvious separation of 160 germplasm materials,but some of the same source of germplasm appeared in clusters.Principal component analysis and cluster analysis verified each other and showed similar results,which may be related to the frequent exchange of seeds and seedlings by the seed growers in different regions.In this study,9 SSR markers associated with 6 phenotypic traits were screened by Chi-square test and T-test,which could be used for marker assisted breeding of female breeding.关键词
知母/表型性状/SSR标记/遗传多样性/关联分析Key words
Anemarrhena asphodeloides/phenotypic traits/SSR markers/genetic diversity/correlation analysis引用本文复制引用
狄根森,闫立萱,任俊男,吴立柱,王泽永,马春英..基于表型性状和SSR标记的知母遗传多样性及关联分析[J].植物遗传资源学报,2025,26(2):271-283,13.基金项目
河北省自然科学基金项目(H2023204001)Natural Science Foundation of Hebei(H2023204001) （H2023204001）
