作物杂志Issue(1)：35-45,11.DOI:10.16035/j.issn.1001-7283.2025.01.005
基于表型性状及SSR标记的扁豆种质资源遗传多样性分析
Genetic Diversity Analysis of Lablab purpureus Germplasm Resources Based on Morphological Trait and SSR Markers
摘要
Abstract
The genetic diversity of 115 Lablab purpureus germplasms from China,India and the United States was analyzed using 17 phenotypic traits and 31 pairs of polymorphic SSR markers.The results showed that a total of 25 types of variation were observed for the nine qualitative traits.The diversity index(H′)ranged from 0.23 to 1.19 with an average of 0.72.The coefficient of variation of eight quantitative traits was 8.97%-36.23%,and most of the phenotypic traits showed rich genetic diversity.Most of genetic information could represented by nine principle component factors with 77.39%of cumulative contribution rate according to principal component analysis(PCA).The 106 alleles were amplified by 31 pairs of SSR primers in L.purpureus germplasms with an average of 1.96 alleles per marker.The polymorphic information content(PIC)of each marker varied from 0.05 to 0.69,and the group genetic diversity indexes ranged from 0.05 to 0.74 with an average of 0.45.The unweighted pair-group method with arithmetic means(UPGMA)cluster analysis divided the L.purpureus germplasms into six groups at the genetic distance of 3.8.Additionally,according to PCA,materials in each group were distributed concentrated while each group was independent to each other.All tested materials were divided into two groups by population genetic structure analysis.There was no consistency between the cluster of L.purpureus germplasms and their geographical origin according to all the UPGMA,PCA and genetic structure analysis.This study demonstrated that L.purpureus germplasms had a high level of genetic diversity.These findings provided scientific basis for the utilization of germplasms resources and breeding of L.purpureus.关键词
扁豆/种质资源/表型性状/SSR标记/遗传多样性Key words
Lablab purpureus/Germplasm resource/Phenotypic trait/SSR marker/Genetic diversity引用本文复制引用
姚陆铭,袁娟,马晓红,王彪..基于表型性状及SSR标记的扁豆种质资源遗传多样性分析[J].作物杂志,2025,(1):35-45,11.基金项目
上海市科技兴农项目(2021-02-08-00-12-F00766) （2021-02-08-00-12-F00766）
