作物杂志Issue(1)：54-65,12.DOI:10.16035/j.issn.1001-7283.2025.01.007
高粱Dof基因家族鉴定及表达分析
Identification and Expression Analysis of Dof Gene Family in Sorghum
摘要
Abstract
DNA binding with one finger(Dof)is a unique transcription factor in plants,which is involved in regulating physiological and biochemical processes such as plant tissue differentiation,seed germination,stress resistance and metabolism.Using a bioinformatics approach,the basic gene structure,physical and chemical characteristics,conserved motifs,promoter cis-acting elements,tissue-specific expression,and drought-stress expression of the sorghum Dof transcription factor family members were examined at the whole genome level in order to identify and examine the members of the family.Following a 20%PEG6000 simulated drought stress treatment of Yuliang 10 seedlings,the expression of Dof gene family members in response to drought stress at various time points was examined using qPCR.Results showed that a total of 30 Dof genes were identified and distributed on nine chromosomes.The results of gene conservation analysis showed that Motif1(zinc finger structure)was the conserved protein sequence of sorghum Dof gene family.Among them,29 genes contained CX2CX21CX2C conserved motif,while the SbDof15 gene was CX20CX2C.The structure of promoter analysis showed that the promoter region of Dof contained ABA,MYB,MeJA,low temperature and other stress response elements.The results of evolutionary tree analysis showed that the homology of sorghum Dof protein was higher than that of maize,and heat map analysis showed that Dof family members were expressed in different parts of sorghum.The results of qRT-PCR indicated that Dof gene family members may be involved in the response of sorghum to drought stress,and different Dof gene members may have different regulatory mechanisms in response to drought stress.关键词
高粱/Dof基因家族/转录因子/全基因组鉴定/PEG处理Key words
Sorghum bicolor/Dof gene family/Transcription factor/Whole genome-wide identification/PEG processing引用本文复制引用
付森杰,宋迎辉,朱灿灿,叶珍言,秦娜,代书桃,刘海霞,王春义,李君霞..高粱Dof基因家族鉴定及表达分析[J].作物杂志,2025,(1):54-65,12.基金项目
中央引导地方科技发展资金(Z20221341070) （Z20221341070）
河南省农业科学院自主创新项目(2023ZC014) （2023ZC014）
农业农村部/财政部现代农业产业技术体系专项资金项目(nycytx-CARS-06) （nycytx-CARS-06）
河南省良种联合攻关项目(2022010401) （2022010401）
河南省农业科学院创新团队专项(2023TD036) （2023TD036）
