作物杂志Issue(1)：83-88,6.DOI:10.16035/j.issn.1001-7283.2025.01.010
突变NtJAZ1基因获得高烟碱烤烟新材料
Mutating NtJAZ1 Gene to Obtain High Nicotine Flue-Cured Tobacco New Material
摘要
Abstract
Nicotine is one of the most important chemical compounds in cultivated tobacco,which largely determines the quality of tobacco leaves.In order to develop a new tobacco material with high nicotine content,the mutant material of NtJAZ1,a negative regulator of nicotine synthesis,was screened from the ethyl methyl sulfonate(EMS)mutant library of Yunyan 87,a major cultivar in China.The results showed that 12 mutant materials were selected from the mutant library,of which ten mutations resulted in the amino acid change of NtJAZ1,one mutation was located in the intron,and one nonsense mutation.The homozygous mutant material was planted in greenhouse.The nicotine contents in leaves of two strains(J8 and J11)were significantly higher than that of the control at bud stage.After topping,the nicotine content of J11 I-1 and J11 IⅤ-1 increased by 48%and 58%,respectively,compared with the control.qRT-PCR showed that the expression levels of NtPMT1a and NtQPT genes in mutant J11 I-1 were significantly increased.In conclusion,the high-nicotine mutant material J11 obtained by screening does not involve transgenic components,which can provide germplasm resources for breeding new varieties of flue-cured tobacco with high nicotine content.关键词
烤烟/烟碱/甲基磺酸乙酯(EMS)/NtJAZ1Key words
Tobacco/Nicotine/Ethyl methyl sulfonate(EMS)/NtJAZ1引用本文复制引用
高玉龙,者开明,赵璐,王丙武,孔光辉,王亚辉,刘剑金,段杰,吴兴富,李青..突变NtJAZ1基因获得高烟碱烤烟新材料[J].作物杂志,2025,(1):83-88,6.基金项目
中国烟草总公司云南省公司科技计划项目(2021530000242029) （2021530000242029）
