中国中西医结合杂志2025，Vol.45Issue(1)：32-41,10.DOI:10.7661/j.cjim.20240902.152
基于16SrRNA测序研究结直肠癌湿热瘀毒证舌苔微生态特征
Study on Micro Ecological Characteristics of Tongue Coating of Colorectal Cancer with Damp-heat Stasis-toxin Syndrome Based on 16SrRNA Sequencing
摘要
Abstract
Objective To explore the biological basis of the damp-heat stasis-toxin syndrome in colorectal cancer(CRC)patients.Methods Totally 100 patients with CRC and 50 healthy individuals were screened.A total of 41 patients with CRC with damp heat stasis syndrome(SRYD),41 patients with CRC without damp heat stasis syndrome(FSRYD)and 40 healthy individuals(Normal)were enrolled.16S rRNA sequencing technology was used to analyze the differences in the composition and diversity of microbial groups.Results There was no significant difference in richness and diversity among SRYD,FSRYD and Normal groups.Compared with other groups,different expressions of Firmicutes,Actinobacteriota,Elusimicrobiota,RCP2-54 at phylum level,Ralstonia,Sphingomonas,Dialister,Corynebacterium,Rothia,Odoribacter,Streptococcus and so on at genus level were detected in SRYD groups.A possible non-invasive biomarker of damp-heat stasis and toxin syndrome in colorectal cancer may be Streptococcus.LEfSe analysis indicated a greater abundance of Firmicutes,Actinobacteriota,Streptococcaceae,Streptococcus,Actinobacteria,Granulicatella,Rothia,Corynebacteriales,Corynebacteriaceae,Corynebacterium in SRYD groups.PICRUSt functional predictive analysis indicated the primary enrichment pathway of the SRYD group's differential expression are phosphotransferase system,valine,leucine and isoleucine degradation,central carbon metabolism in cancer,carbohydrate transport and metabolism.Conclusions It is reasonable to analyze CRC Chinese medicine syndrome differentiation from the perspective of tongue coating flora.Tongue coating flora can be used to diagnose damp-heat stasis-toxin syndrome in CRC patients.Streptococcus could be a noninvasive biomarker for damp-heat stasis-toxin syndrome in CRC.关键词
结直肠癌/舌象/舌苔菌群/中医证型/16SrRNA测序/中西医结合Key words
colorectal cancer/tongue image/oral microbiota/Chinese medicine syndrome/16SrRNA sequencing/integrative medicine引用本文复制引用
徐思源,程海波,张钦畅,耿雪尘,孙珍珍,倪明欣,惠逸帆,孙晓荷,方妍,李柳..基于16SrRNA测序研究结直肠癌湿热瘀毒证舌苔微生态特征[J].中国中西医结合杂志,2025,45(1):32-41,10.基金项目
国家中医药管理局中医药创新团队及人才支持计划项目(No.ZYYCXTD-C-202208) （No.ZYYCXTD-C-202208）
国家中医药管理局高水平中医药重点学科建设项目(No.国中医药人教函[2023)85号) （No.国中医药人教函[2023）
国家自然科学基金资助项目(No.81930117) （No.81930117）
江苏高校优势学科建设工程资助项目(No.苏政协发[2018]87号) （No.苏政协发[2018]87号）
