中药新药与临床药理2025，Vol.36Issue(2)：161-167,7.DOI:10.19378/j.issn.1003-9783.2025.02.001
槲皮素调节JAK2/STAT3信号通路对早发性卵巢功能不全大鼠卵巢功能损伤的影响
Effect of Quercetin on Ovarian Function Damage in Rats with Early-onset Ovarian Insufficiency by Regulating the JAK2/STAT3 Signaling Pathway
摘要
Abstract
Objective To investigate the effect of quercetin on improving ovarian function injury in rats with early-onset ovarian insufficiency by regulating JAK2/STAT3 signaling pathway.Methods The rat model of early-onset ovarian insufficiency was constructed and randomly divided into model group,quercetin low-dose(25 mg·kg-1)group,quercetin medium-dose(50 mg·kg-1)group,quercetin high-dose(100 mg·kg-1)group,quercetin high-dose+JAK2 pathway activator CA1(100 mg·kg-1 quercetin+4 mg·kg-1 CA1)group,with 10 rats in each group.Another 10 healthy rats were selected as control group.The ovarian index,serum levels of follicle-stimulating hormone(FSH),anti-Müllerian hormone(AMH),estradiol(E2),malondialdehyde(MDA),superoxide dismutase(SOD)and catalase(CAT)were detected in all rats.Pathological changes of ovarian tissue and the number of follicles in different stages of development(primary,preantral,antrum,mature and atretic follicles)were observed by HE staining.The apoptosis of ovarian granulosa cells was observed by TUNEL staining and the protein expression of JAK2/STAT3 signaling pathway was detected by Western Blot.Results Compared with the control group,the model group had more severe ovarian lesions.A large number of cells were lost,and the overall structure was destroyed seriously.The number of atretic follicles increased,whereas the number of other follicles decreased.The levels of ovarian index,sex hormones(AMH and E2),oxidative stress indexes(SOD and CAT)decreased significantly,and the levels of MDA and FSH increased significantly.The apoptotic rate of ovarian granulosa cells increased,and the protein expressions of p-JAK2/JAK2 and p-STAT3/STAT3 were significantly increased(P<0.05).Compared with model group,with the increase of quercetin dose,the degree of ovarian lesion of rats in quercetin low-,medium-and high-dose groups was significantly reduced,cells gradually restored to order,the number of atretic follicles decreased,but the number of other follicles increased.The levels of ovarian index,sex hormone(AMH and E2)and oxidative stress index(SOD and CAT)were significantly increased,the levels of MDA and FSH were significantly decreased,the apoptotic ratio of ovarian granulosa cells was decreased,and the protein expressions of p-JAK2/JAK2 and p-STAT3/STAT3 were significantly decreased(P<0.05).Compared with quercetin high-dose group,CA1 could reverse the improvement effect of quercetin on ovarian injury in rats with early-onset ovarian insufficiency in quercetin high-dose+CA1 group(P<0.05).Conclusion Quercetin can alleviate oxidative stress,reduce cell apoptosis,improve ovarian damage and enhance ovarian protection,which may be related to inhibiting JAK2/STAT3 signaling pathway.关键词
槲皮素/早发性卵巢功能不全/卵巢损伤/JAK2/STAT3信号通路/大鼠Key words
Quercetin/early-onset ovarian insufficiency/ovarian damage/JAK2/STAT3 signaling pathway/rats分类
医药卫生引用本文复制引用
江银,丁怡,于潇,王徽..槲皮素调节JAK2/STAT3信号通路对早发性卵巢功能不全大鼠卵巢功能损伤的影响[J].中药新药与临床药理,2025,36(2):161-167,7.基金项目
国家自然科学基金项目(82104917). （82104917）
