中药新药与临床药理2025，Vol.36Issue(2)：168-174,7.DOI:10.19378/j.issn.1003-9783.2025.02.002
血府逐瘀汤调控cAMP/PKA/PPARγ信号通路对动脉粥样硬化大鼠炎性损伤的影响
Effect of Xuefu Zhuyu Decoction on Inflammatory Injury in Atherosclerotic Rats by Regulating cAMP/PKA/PPARγ Signaling Pathway
摘要
Abstract
Objective To explore the effect of Xuefu Zhuyu Decoction on inflammatory injury of aorta in atherosclerotic(AS)rats based on cAMP/PKA/PPARγ pathway.Methods A high-fat diet combined with intraperitoneal injection of vitamin D3 was used to establish an AS rat model.After being modeled,AS rats were stochastically assigned into a model group,H-89 group(5 mg·kg-1),Xuefu Zhuyu Decoction group(12 g·kg-1),and Xuefu Zhuyu Decoction+H-89 group(12 g·kg-1 Xuefu Zhuyu Decoction+5 mg·kg-1 H-89),with 12 rats in each group.Another 12 normal rats were regarded as the control group.After 12 weeks of administration,the levels of serum lipids and inflammatory factors were measured with the reagent kits.Oil red O staining was applied to observe lipid deposition in the aorta.HE staining was applied to observe pathological changes in the aorta.ELISA method was applied to detect cAMP level in aortic tissue.Western Blot was applied to detect the protein expression of PKA and PPARγ in rat aortic tissue.Results Compared with the control group,the levels of triglyceride(TC),total cholesterol(TG),low density lipoprotein cholesterol(LDL-C),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in serum,the proportion of aortic plaque injury,and the intima-media thickness in the model group were increased,while the level of high density lipoprotein cholesterol(HDL-C)in serum,cAMP level,protein levels of PKA and PPARγ in the aortic tissue were decreased(P<0.05).Compared with the model group,the levels of TC,TG,LDL-C,IL-6,IL-1β,and TNF-α in serum,the proportion of aortic plaque injury,and the intima-media thickness in Xuefu Zhuyu Decoction group were decreased,while HDL-C level in serum,cAMP level and protein levels of PKA and PPARγ in the aortic tissue were increased(P<0.05).The above indicators in the H-89 group showed opposite changes(P<0.05).H-89 could weaken the improvement effect of Xuefu Zhuyu Decoction on aortic injury in AS rats.Conclusion Xuefu Zhuyu Decoction may improve dyslipidemia,inhibit inflammatory response,and alleviate arterial inflammatory damage in AS rats by activating the cAMP/PKA/PPARγ pathway.关键词
血府逐瘀汤/动脉粥样硬化/主动脉炎性损伤/环磷酸腺苷/蛋白激酶A/过氧化物酶体增殖物激活受体γ/大鼠Key words
Xuefu Zhuyu Decoction/atherosclerosis/arterial inflammatory injury/cyclic adenosine monophosphate/protein kinase A/peroxisome proliferator activated receptor γ/rats分类
医药卫生引用本文复制引用
武洪方,于静,袁晓璞,杨丽丽..血府逐瘀汤调控cAMP/PKA/PPARγ信号通路对动脉粥样硬化大鼠炎性损伤的影响[J].中药新药与临床药理,2025,36(2):168-174,7.基金项目
河北省中医药管理局科研项目(2022425). （2022425）
