中药新药与临床药理2025，Vol.36Issue(2)：182-196,15.DOI:10.19378/j.issn.1003-9783.2025.02.004
蒙药嘎拉图呼和调控肿瘤微环境抗胃癌作用机制研究
Study on the Mechanism of Mongolian Medicine Galatu Huhe in Regulating the Tumor Microenviron⁃ment Against Gastric Cancer
摘要
Abstract
Objective To reveal the potential targets and regulatory networks of the Mongolian medicine Galatu Huhe(GLTHH)in the treatment of gastric cancer,and to provide important data support for the in-depth research and clinical application of GLTHH and offer a new research perspective for Mongolian medicine in the treatment of gastric cancer.Methods HPLC-Q-Exactive MS was used to rapidly and accurately analyze and identify the chemical components of GLTHH,as well as to determine its potential pharmacological chemical component groups and elucidate their fragmentation patterns.Active chemical components were screened using the TCMSP database based on mass spectrometry data.Their potential targets were identified using the Pharm Mapper database.The data of human gastric cancer and normal tissue were collected from the GEO database for differential gene screening and immune cell infiltration analysis to obtain disease-related genes and immune cells.The intersection of disease genes and potential targets of GLTHH was taken to obtain key therapeutic targets,which were then analyzed for their correlation with immune cells to identify core therapeutic targets.Finally,molecular docking was employed to verify binding capabilities.Results A total of 105 compounds were identified in GLTHH,and 658 potential targets were obtained.After correction of GEO data,206 differentially expressed genes(DEGs)were identified.The intersection of DEGs with the potential targets of GLTHH yielded eight key target genes.Immune infiltration analysis showed that,compared with the normal group,NK cells were significantly decreased in the gastric cancer group.Among the key target genes,15-hydroxyprostaglandin dehydrogenase(HPGD)was positively correlated with NK cells,while transglutaminase 2(TGM2)was negatively correlated with NK cells.Conclusion GLTHH may achieve therapeutic effects by regulating the tumor microenvironment and blocking immunosuppressive effect of NK cells induced by gastric cancer cells.关键词
蒙药/嘎拉图呼和/胃癌/HPLC-Q-Exactive MS/免疫逃逸/肿瘤微环境Key words
Mongolian medicine/Galatu Huhe/gastric cancer/HPLC-Q-Exactive MS/immune escape/tumor microenvironment分类
医药卫生引用本文复制引用
刘静,李明钦,王梓瑄,张谦,李君,张微,王芳,王跃武..蒙药嘎拉图呼和调控肿瘤微环境抗胃癌作用机制研究[J].中药新药与临床药理,2025,36(2):182-196,15.基金项目
内蒙古自治区自然科学基金项目(2023QN08050) （2023QN08050）
内蒙古自治区高等学校科学研究项目(NJZY23135). （NJZY23135）
