中国现代中药2025，Vol.27Issue(2)：202-209,8.DOI:10.13313/j.issn.1673-4890.20240913001
基于LNA-TaqMan探针实时荧光定量PCR检测技术的天山雪莲掺伪研究
Research on Adulteration of Saussurea involucrata Based on Real-time Fluorescence Quantitative PCR Detection of LNA-TaqMan Probe
摘要
Abstract
Objective:To establish a highly efficient and rapid method for identifying Saussurea medusa and S.lanicepsin in S.involucrata by using locked nucleic acid(LNA)-TaqMan real-time fluorescence quantitative polymerase chain reaction(PCR)technology.Methods:Based on the real-time fluorescence quantitative PCR technology of the LNA-TaqMan probe,the chloroplast genome sequence differences of S.involucrata and its common adulterants S.medusa and S.laniceps were utilized to design and screen probe primers based on the specific differences among S.medusa,S.laniceps,and S.involucrata.The specificity of primers and LNA-TaqMan probe was verified,and the adulteration ratio was calculated based on the difference in critical cycle number(Ct)values of amplification curves.Results:Based on the LNA-TaqMan probe detection method,S.medusa and S.laniceps were detected by the appearance of specific amplification curves.The adulteration ratio was effectively determined by the difference in the Ct values of the amplification curves,and stable detection could still be achieved at an adulteration ratio of 1%.Conclusion:This method has high sensitivity and specificity for detecting adulterants S.medusa and S.laniceps in S.involucrata and can effectively solve the problem of difficult identification of S.involucrata adulterants,providing technical support for the quality control of S.involucrata medicinal materials.关键词
天山雪莲/水母雪兔子/绵头雪兔子/锁核酸-TaqMan探针/实时荧光定量聚合酶链式反应/掺伪Key words
Saussurea involucrata(Kar.et Kir.)Sch.-Bip./Saussurea medusa Maxim./Saussurea laniceps Hand.-Mazz./LNA-TaqMan probe/real-time fluorescent quantitative polymerase chain reaction/adulteration分类
医药卫生引用本文复制引用
王多梅,杨建波,杨乐,于新兰,李海芳,胡冲,陈灵丽,蒲婧哲,张亚中..基于LNA-TaqMan探针实时荧光定量PCR检测技术的天山雪莲掺伪研究[J].中国现代中药,2025,27(2):202-209,8.基金项目
安徽省药品监督管理局科技创新项目(AHYJ-KJ-202313) （AHYJ-KJ-202313）
新疆维吾尔自治区智力援疆创新拓展人才计划项目 （）
