中国现代中药2025，Vol.27Issue(2)：242-247,6.DOI:10.13313/j.issn.1673-4890.20241016002
基于DSS标记鉴别牡丹、芍药及川赤芍
Identification of Paeonia suffruticosa,Paeonia lactiflora,and Paeonia veitchii Based on DNA Signature Sequence Tags
摘要
Abstract
Objective:To establish a quick,convenient,and accurate DNA signature sequence tag(DSS)-based molecular identification method to identify Paeonia suffruticosa,P.lactiflora,and P.veitchii.Methods:DSS groups and their specific primers were screened by using IdenDSS software based on the chloroplast genome database of plants in Paeonia L..After DNA extraction,polymerase chain reaction(PCR)amplification,sequencing,and sequence alignment,the target DSSs used to validate P.suffruticosa,P.lactiflora,and P.veitchii were obtained.Results:The verification results of five candidate DSSs determined that two DSSs(GTAACTTGCTATCCTCTTGCCTAGCAGGCAAAGATTTACT and ACTACGAAGAAGTTCCGGGGGTTACAAAGGAAACTTCGAG)could be used for the identification of P.suffruticosa,P.lactiflora,and P.veitchii.The DSSs were uploaded to the Traditional Chinese Medicine Molecular Identification Platform(http://www.herbsdna.com).With the help of the BLAST function,a rapid and accurate identification of P.suffruticosa,P.lactiflora,and P.veitchii was achieved.Conclusion:The screened DSSs could be effectively applied to the origin identification of P.suffruticosa,P.lactiflora,and P.veitchii.关键词
牡丹/芍药/川赤芍/DNA特征序列/基原鉴定Key words
Paeonia suffruticosa Andr./Paeonia lactiflora Pall./Paeonia veitchii Lynch/DNA signature sequence/original identification分类
医药卫生引用本文复制引用
陈灵丽,王多梅,蒲婧哲,胡冲,蒋超,袁媛,张亚中..基于DSS标记鉴别牡丹、芍药及川赤芍[J].中国现代中药,2025,27(2):242-247,6.基金项目
国家药品监督管理局中成药质量评价重点实验室开放课题(2024-KFKT-002) （2024-KFKT-002）
安徽省药品监督管理局监管科学研究重点项目(AHYJ-KJ-202308) （AHYJ-KJ-202308）
安徽省药品监督管理局科技创新项目(AHYJ-KJ-202313) （AHYJ-KJ-202313）
