中国现代中药2025，Vol.27Issue(2)：248-257,中插1-中插6,16.DOI:10.13313/j.issn.1673-4890.20240905004
基于非靶向组学结合化学计量学方法分析新疆紫草差异成分
Analysis of Differential Metabolites of Arnebia euchroma Based on Non-targeted Metabolomics Combined with Chemometrics
摘要
Abstract
Objective:To investigate the differential metabolites of Arnebia euchroma from different producing areas by non-targeted metabolomics and provide reference for the development and utilization of A.euchroma resources.Methods:Ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was employed to analyze the metabolomes of A.euchroma samples from different producing areas and identify the chemical components based on the characteristic peaks and the database matching.Multivariate statistical analysis was performed to comprehensively analyze the chemical components of A.euchroma samples from different producing areas.Results:A total of 840 compounds were identified in A.euchroma samples from different producing areas,and 262 differential metabolites were screened out by multivariate statistical analysis,including 53 flavonoids,52 alkaloids,47 terpenoids,24 phenols and phenolic acids,7 fatty acyls,and 6 coumarins.A total of 703 common compounds were detected in A.euchroma samples from different producing areas,among which quinones,amides,and amino acid derivatives were detected in two different modes,with high relative content.Erucic amide had the highest relative content,followed by the amino acid derivative L-proline.Pathway enrichment analysis showed that the differential metabolites mainly involved 12 metabolic pathways,including flavonoid and flavonol biosynthesis,purine metabolic pathway,pantothenic acid and coenzyme A(CoA)biosynthesis,aminoacyl-tRNA biosynthesis,pyrimidine biosynthesis,and amino acid biosynthesis pathway.Conclusion:There are obvious differences in the metabolites of A.euchroma from different producing areas,and 12 compounds including alkannin,chlorogenic acid,and kaempferol can be used as metabolic markers of A.euchroma from different producing areas,which can provide reference for the quality evaluation and resource utilization of A.euchroma.关键词
新疆紫草/液相色谱-质谱法/差异代谢物/非靶向组学/化学计量学方法Key words
Arnebia euchroma(Royle)Johnst./liquid chromatography-mass spectrometry/differential metabolites/non-targeted metabolomics/chemometrics分类
医药卫生引用本文复制引用
叶雨,康传志,张际昭,邱远金,赵亚琴,王升,李晓瑾,王果平,樊丛照..基于非靶向组学结合化学计量学方法分析新疆紫草差异成分[J].中国现代中药,2025,27(2):248-257,中插1-中插6,16.基金项目
新疆维吾尔自治区重大科技专项(2023A03005-1) （2023A03005-1）
中央本级重大增减支项目(2060302) （2060302）
"天山英才"医药卫生高层次人才培养计划(TSYC202301B054) （TSYC202301B054）
贵州省科技计划项目(黔科合服企[2023]012) （黔科合服企[2023]012）
