法医学杂志2024，Vol.40Issue(6)：569-574,6.DOI:10.12116/j.issn.1004-5619.2024.340606
采用LC-HRMS分析米氮平在人肝微粒体中的体外代谢产物
Analysis of In Vitro Mirtazapine Metabolites in Human Liver Microsomes by LC-HRMS
张瑛 1张文芳 1徐多麒 2覃仕扬 1杨士云 1乔静1
作者信息
- 1. 北京市公安司法鉴定中心 法庭毒物分析公安部重点实验室,北京 100192
- 2. 司法鉴定科学研究院 上海市法医学重点实验室 司法部司法鉴定重点实验室 上海市司法鉴定专业技术服务平台,上海 200063
- 折叠
摘要
Abstract
Objective To establish and optimize an in vitro incubation system with human liver micro-somes and investigate the in vitro metabolites and possible metabolic pathways of mirtazapine.Methods Three major metabolites of mirtazapine were selected to optimize the incubation conditions of liver mi-crosomes.The metabolites of mirtazapine were analyzed by liquid chromatography-high resolution mass spectrometry(LC-HRMS)to identify the in vitro metabolites and metabolic pathways of mir-tazapine.Results Ten metabolites,including nine phase Ⅰ metabolites and one phase Ⅱ metabolite,were identified in the in vitro liver microsome incubation.Among them,five new metabolites and one new metabolic pathway were discovered.The pathways involved in phase Ⅰ metabolic included methyla-tion,hydroxylation,oxidation,reduction,etc.,while the phase Ⅱ biotransformation was mainly glucuroni-dation.Conclusion The metabolites discovered in this study are consistent with the main metabolites of mirtazapine reported in literature,which are N-desmethylmetazapine,8-hydroxy mirtazapine and mirtazapine-N-oxide.The results can provide basis for the confirmation of mirtazapine cases and pro-vide reference for the study of other substances.关键词
法医学/毒物分析/米氮平/人肝微粒体/代谢产物/代谢途径/液相色谱-高分辨质谱法(LC-HRMS)Key words
forensic medicine/toxicological analysis/mirtazapine/human liver microsome/metabolite/metabolic pathway/liquid chromatography-high resolution mass spectrometry(LC-HRMS)分类
医药卫生引用本文复制引用
张瑛,张文芳,徐多麒,覃仕扬,杨士云,乔静..采用LC-HRMS分析米氮平在人肝微粒体中的体外代谢产物[J].法医学杂志,2024,40(6):569-574,6.
