吉林医药学院学报2025，Vol.46Issue(1)：15-18,4.
乌拉尔甘草GubHLH基因克隆与原核表达
Cloning and Prokaryotic Expression of GubHLH Gene in Glycyrrhiza Uralensis Fisch
摘要
Abstract
Objective To construct a prokaryotic expression vector of the basic helix loop helix(GubHLH)transcription factor in licorice(Glycyrrhiza uralensis Fisch.)and prokaryotic expression was performed and identified.Methods The total mRNA was extracted from licorice root,and reverse transcription polymerase chain reaction was performed to amplify the full length of GubHLH gene.The prokary-otic expression vector pET28a-GubHLH was constructed and the optimal IPTG concentration,temperature,and time conditions for Gub-HLH expression were analyzed.Results The optimal induction condition combination of obtained recombinant plasmid pET28a-GubHLH in E.coli BL21(DE3)is to induce the target protein with 0.2 mmol/L IPTG at 37℃ for 3 hours.Conclusion The prokaryotic expression system of GubHLH protein is constructed successfully.The optimal induction condition combination of obtained recombinant plasmid pET28a-GubHLH in E.coli BL21(DE3)is confirmed.关键词
甘草/原核表达/bHLH基因Key words
licorice(Glycyrrhiza uralensis Fisch.)/prokaryotic expression/bHLH gene分类
中医学引用本文复制引用
张艺朦,付业繁,赫苯玎,陈晓芳,孙欣,李欣,方圆..乌拉尔甘草GubHLH基因克隆与原核表达[J].吉林医药学院学报,2025,46(1):15-18,4.基金项目
吉林医药学院大学生创新创业训练计划项目(2023CXXL028) （2023CXXL028）
