福建农业学报2024，Vol.39Issue(12)：1346-1353,8.DOI:10.19303/j.issn.1008-0384.2024.12.004
番鸭腺病毒的分离鉴定及hexon基因遗传变异研究
Identification and Hexon Gene Variation of Muscovy Duck Adenovirus
摘要
Abstract
[Objective]Characteristics and epidemiology of the muscovy duck white hepatitis in Fujian were studied,and genetic variations of the pathogen capsid protein determined.[Methods]Clinical specimens of 9 suspected muscovy duck white hepatitis cases incurred at Fuzhou,Sanming,Putian,and Zhangzhou in the province were collected to test for DAdVs using pathogen detection methods.Isolated pathogens were identified,and their hemagglutination characteristics,pathogenicity,and hexon gene sequences determined.[Results]The disease was found on muscovy ducks mostly in spring and autumn with an incidence rate of 15%-40%and a mortality rate of 5%-15%.The DAdVs were isolated and tested to be lethal to muscovy duck embryos.The infection rate of the virus on ducks ranged 60%-100%with a mortality rate of 20%-40%.The homology of the hexon gene nucleotides among the 9 isolated DAdVs was greater than 99.9%.The capsid proteins had sequences similar to that of CH-GD-12-2014 and were stable proteins without base deletion,insertion or recombination.The 9 isolates had a 99.9%-100%homology with CH-GD-12-2014 from Guangdong,76.6%-76.7%with DAdV-GR from France,and 64.1%-66.8%with FAdV and other serotypes.They were in the same branch with GD-12-2014 and GR,but not AdV,on the phylogenetic tree.[Conclusion]The strains of the muscovy duck adenovirus circulating in Fujian Province in 2022 belonged to the DAdV Type 2.关键词
番鸭腺病毒/流行病学/病原/hexon基因Key words
muscovy duck adenovirus/epidemiology/pathogen/hexongene分类
农业科技引用本文复制引用
林锋强,陈小丽,李典辉,沈华伟,董慧,朱小丽,王劭..番鸭腺病毒的分离鉴定及hexon基因遗传变异研究[J].福建农业学报,2024,39(12):1346-1353,8.基金项目
福建省科技计划公益类专项(2024R1025007) （2024R1025007）
福建省星火计划项目(2022S0011) （2022S0011）
