福建农业学报2024，Vol.39Issue(12)：1354-1364,11.DOI:10.19303/j.issn.1008-0384.2024.12.005
杧果果肉多酚积累关键酶基因鉴定与序列分析
Identification and Sequences of Key Polyphenol-synthesis Genes in Mango Pulp
摘要
Abstract
[Objective]Genes of phenylalanine ammonia lyase(MiPAL)and polyphenol oxidase(MiPPO)syntheses in Mangifera indica L.were identified with their regulation functions on fruit growth and development determined.[Methods]Using bioinformatics methods and mango genomes,the PAL and PPO family members in the fruits were identified based on analyses of protein characteristics,phylogenetic relationship,gene structure,and promoter cis acting elements.Expressions of the identified genes during fruit development were determined by transcriptome analysis.[Results]Ten PALs and one PPO in the mangos were identified.They were stable,hydrophilic proteins located mainly in the chloroplasts.Being a woody plant,these genes are phylogenetically in a same subgroup of 4 PAL and PPO categories.The sequences of 25%of MiPALs lacked introns,and that of MiPPO without intron at all.The 10 MiPALs were distributed in 5 chromosomes and one fragment with 11 pairs of segmental duplication genes and 7 tandem duplication genes.Located in chromosome Chr12,MiPPO was homologous to PPO13 in Populus tomentosa.The cis acting elements in the promoters of MiPAL and MiPPO contained those of growth,development,and hormone and stress responses expressed in an inducible pattern.MiPALs were highly expressed in the fruits at both young and mature stages,whereas MiPPO only at maturation.[Conclusion]With the information obtained,further exploration on the molecular regulatory mechanisms and biological functions of polyphenol accumulation in mango pulp was in order.关键词
杧果/多酚积累/基因家族/基因表达Key words
Mangifera indica L./polyphenol synthesis/gene family/gene expression分类
农业科技引用本文复制引用
施绍璞,周开兵..杧果果肉多酚积累关键酶基因鉴定与序列分析[J].福建农业学报,2024,39(12):1354-1364,11.基金项目
国家自然科学基金项目(32160677) （32160677）
海南省自然科学基金项目(321RC465) （321RC465）
