福建农业学报2024，Vol.39Issue(12)：1402-1411,10.DOI:10.19303/j.issn.1008-0384.2024.12.010
野生中国沙棘根际假单胞属菌株的筛选鉴定及其对雍菜促生效果的影响
Pseudomonas in Hippophae rhamnoides Rhizosphere Affecting Growth of Ipomoea aquatica
摘要
Abstract
[Objective]Pseudomonas sp.in the rhizosphere of Hippophae rhamnoides were isolated and studied for potential application as a biofertilizer.[Method]Microorganisms in the rhizosphere soil of Hippophae rhamnoides sinensis subsp.in the wild in Qinghai Province were isolated by using selected media and purified by plate streaking.Candidate Pseudomonas strains were morphologically,physiologically,and biochemically identified as well as 16S rDNA sequenced.Abilities of the isolates to degrade organic and inorganic phosphorus,potassium,cellulose and/or to fix nitrogen were examined.Effects of spraying the bacterial culture broth of the individual isolates on the seed germination and seedling growth of Ipomoea aquatica Forssk were observed.[Result]On different media of specific formulations,the diameters of the turbid circles born by the 7 isolated Pseudomonas strains cultured for 3 d ranged 4.28-13.71 mm with 5.15-25.41 μg·mL-1 of dissolved organic phosphorus,those of clear circles 3.51-7.62 mm with 2.15-22.26 μg·mL-1 of dissolved inorganic phosphorus,those of halos 11.12-21.85 mm with 5.07-14.33 μg·mL-1 of dissolved potassium,those of transparent circles 4.61-10.22 mm of cellulose-degradation,and the ratios of the nitrogen-fixing clear circle diameter(D)to the colony growth circle diameter(d)1.33-1.86.The isolated Pseudomonas strains significantly improved the I.aquatica seed germination rate and seedling growth.Among them,ZGSJ-3 showed the largest increases of 35.2%on the 3.69 mm leaf width and of 41.2%on the 50.25 mm stem length over control.[Conclusion]Presence of the Pseudomonas sp.,especially ZGSJ-3 and ZGSJ-7,isolated in this study significantly improved the seed germination and seedling growth of I.aquatica.关键词
中国沙棘/雍菜/假单胞菌/根际促生菌/促生能力Key words
Hippophae rhamnoides sinensis subsp./Ipomoea aquatica Forssk./Pseudomonas sp./rhizosphere growth-promoting bacteria/growth-promoting capacity分类
农业科技引用本文复制引用
高佩,徐淑琴,贺曦,三鸿源,马玉花,冶贵生..野生中国沙棘根际假单胞属菌株的筛选鉴定及其对雍菜促生效果的影响[J].福建农业学报,2024,39(12):1402-1411,10.基金项目
青海省"高端创新人才千人计划"项目(2022) （2022）
