福建农业学报2024，Vol.39Issue(12)：1412-1420,9.DOI:10.19303/j.issn.1008-0384.2024.12.011
酒糟源酶解产物对黄嘌呤氧化酶的抑制作用及酶解工艺优化
Inhibition of Xanthine Oxidase by Enzymatic Hydrolysates of Distiller's Grains and Optimization of Enzymatic Hydrolysis Process
摘要
Abstract
[Objective]To investigate the inhibitory effects of enzymatic hydrolysates from different distiller's grain proteins on xanthine oxidase(XOD)activity,providing a theoretical basis and technical support for the development of XOD inhibitory peptides from distiller's grains.[Methods]Using Hongqu distiller's grains(HDG)and sauce-flavor Baijiu distiller's grains(BDG)as raw materials,alkaline protease was used as the hydrolytic enzyme.The protein hydrolysis rate,XOD inhibitory activity of the hydrolysates,and their tolerance to simulated gastrointestinal digestion were compared.The optimal distiller's grain source was selected for enzymatic hydrolysis process optimization.[Results]1)The enzymatic hydrolysates of both HDG and BDG exhibited XOD inhibitory activity.BDG had a higher protein hydrolysis rate(56.28±0.01)%and stronger XOD inhibitory activity,with a half-maximal inhibitory concentration(IC50)of(7.73±0.26)mg·mL-1,showing significant differences compared to HDG(P＜0.01).2)After gastrointestinal digestion,the XOD inhibitory activity retention rates of the hydrolysates from both distiller's grains were above 31%,with no significant difference between them(P＞0.05).3)The optimized enzymatic hydrolysis conditions for BDG were:alkaline protease dosage of 6000 U·g-1,temperature of 50℃,pH 9.5,and time of 5 h.Under these conditions,the protein hydrolysis rate was 58.00%,and the IC50 of the hydrolysates for XOD inhibition was 5.54 mg·mL-1.[Conclusion]The enzymatic hydrolysates of sauce-flavor Baijiu distiller's grains(BDG)exhibit good XOD inhibitory activity.BDG is a promising protein source for the preparation of XOD inhibitory peptides.关键词
酒糟/酶解产物/酶解率/黄嘌呤氧化酶/半抑制浓度/工艺优化Key words
distiller's grains/enzymatic hydrolysates/enzymatic hydrolysis rate/xanthine oxidase/half-maximal inhibitory concentration(IC50)/process optimization分类
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王瑛,林晓婕,张甜甜,贾淑宁,梁璋成,林河通,何志刚..酒糟源酶解产物对黄嘌呤氧化酶的抑制作用及酶解工艺优化[J].福建农业学报,2024,39(12):1412-1420,9.基金项目
福建省科技计划公益类专项(2024R11030089、2024R1031002) （2024R11030089、2024R1031002）
